State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Talanta. 2013 Oct 15;115:1-5. doi: 10.1016/j.talanta.2013.03.073. Epub 2013 Apr 15.
In this work, with the assistance of the additivity of surface plasmon resonance band of nonionic fluorosurfactant-stabilized gold nanoparticles, we developed a new colorimetric assay approach of cysteine (Cys) and homocysteine (Hcy) in biological fluids, requiring no use of separation techniques. The detection limits of Cys and Hcy can be as low as 0.4 μM. The applicability of the method was validated by spiking known amount of Cys and Hcy in human urine and plasma samples. Recoveries were in the range of 97.2-106.7%. The present approach is simple, high selective and reproducible. In addition, the universality of the additivity of surface plasmon resonance band was demonstrated by virtue of silver nanoparticles. Therefore, the proposed method has a great potentiality in disease diagnosis associated with Cys and Hcy.
在这项工作中，我们借助非离子型氟表面活性剂稳定的金纳米粒子表面等离子体共振带的加和性，开发了一种新的无需使用分离技术即可用于生物体液中半胱氨酸（Cys）和高半胱氨酸（Hcy）的比色分析方法。该方法对 Cys 和 Hcy 的检测限可低至 0.4 μM。通过向人尿液和血浆样品中加入已知量的 Cys 和 Hcy 对该方法的适用性进行了验证。回收率在 97.2-106.7%范围内。该方法简单、选择性高、重现性好。此外，通过银纳米粒子证明了表面等离子体共振带加和性的通用性。因此，该方法在与 Cys 和 Hcy 相关的疾病诊断方面具有很大的潜力。
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