一种测定生物样品中乐卡地平的新伏安法。
A new voltammetric method for the determination of lercanidipine in biological samples.
作者信息
Oztürk Funda, Taşdemir Ibrahim Hüdai, Erdoğan Deniz Altunöz, Erk Nevin, Kılıç Esma
出版信息
Acta Chim Slov. 2011 Dec;58(4):830-9.
Electrochemical behavior and adsorption-diffusion properties of lercanidipine (LCN) on a glassy carbon electrode (GCE) were investigated in a mixture of ethanol-Britton Robinson buffer (BR) using voltammetric methods. From experimental results LCN was found to be reduced irreversibly via a single four-electron process controlled mainly by diffusion with some adsorption contribution at about -0.65 V (vs. Ag/AgCl reference electrode). Therefore, a new, accurate, rapid, selective and simple square-wave cathodic adsorptive stripping voltammetric (SWCAdSV) method could be developed for direct determination of LCN in pharmaceutical preparations, spiked human urine and spiked human serum samples without time-consuming steps prior to drug assay. The peak current of the reduction wave linearly changed with the concentration of LCN in the concentration range between 4.0 × 10-8 molL-1 and 7.6 × 10-6 molL-1 in two different regions where optimum preconcentration potential and optimum preconcentration time were applied as -0.20 V and 90 s, respectively. The limit of detection (LOD) and the limit of quantitation (LOQ) values were found to be 2 × 10-8 molL-1 (0.01 mgL-1) and 6 × 10-8 molL-1 (0.04 mgL-1), respectively. The method was applied to determine the content of LCN in commercial pharmaceutical preparation, spiked human serum and spiked human urine. The method was found to be highly accurate and precise, having a relative standard deviation of less than 10% in all applications.
采用伏安法研究了乐卡地平（LCN）在玻碳电极（GCE）上于乙醇 - Britton Robinson缓冲液（BR）混合体系中的电化学行为及吸附 - 扩散性质。实验结果表明，LCN在约 -0.65 V（相对于Ag/AgCl参比电极）处通过主要受扩散控制且有一定吸附作用的单四电子过程不可逆还原。因此，可开发一种新的、准确、快速、选择性好且简便的方波阴极吸附溶出伏安法（SWCAdSV），用于直接测定药物制剂、加标人尿和加标人血清样品中的LCN，无需在药物分析前进行耗时的步骤。在两个不同区域，当最佳预富集电位和最佳预富集时间分别为 -0.20 V和90 s时，还原波的峰电流在4.0×10⁻⁸ molL⁻¹至7.6×10⁻⁶ molL⁻¹的浓度范围内随LCN浓度线性变化。检测限（LOD）和定量限（LOQ）值分别为2×10⁻⁸ molL⁻¹（0.01 mgL⁻¹）和6×10⁻⁸ molL⁻¹（0.04 mgL⁻¹）。该方法用于测定市售药物制剂、加标人血清和加标人尿中LCN的含量。结果表明该方法具有高准确性和精密度，在所有应用中的相对标准偏差均小于10%。
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