Elizondo-Zertuche Mariana, Robledo-Leal Efrén, González J Gerardo, Ceceñas Luis A, González Gloria M
Universidad Autónoma de Nuevo León, UANL, Facultad de Medicina, Departamento de Microbiología, Av. Francisco I. Madero y calle Dr. E. A. Pequeño S/N, Colonia Mitras Centro, Monterrey, Nuevo León, C.P. 64460, Mexico.
Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Biológicas, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza, Nuevo León, C.P. 66451, Mexico.
Rev Iberoam Micol. 2015 Jan-Mar;32(1):30-3. doi: 10.1016/j.riam.2013.09.006. Epub 2013 Sep 23.
The incidence of vulvovaginal candidiasis, a common infection among healthy women primarily caused by the yeast Candida albicans, has increased significantly in recent years.
The purpose of this study was to compare the efficacy of ravuconazole (RVC) and fluconazole (FLC) in the treatment of experimental C. albicans vaginitis.
Forty isolates of C. albicans were screened for their in vitro susceptibility to RVC and FLC. A strain of C. albicans that was resistant to FLC (minimum inhibitory concentration [MIC] of >64 μg/ml) was selected for the in vivo study. Treatment regimens for the murine vaginal infection model were (1) 1, 5, 10, and 20 mg/kg RVC once daily, (2) 20 mg/kg RVC twice daily, (3) 20 mg/kg FLC once daily, and (4) 20 mg/kg FLC twice daily.
The geometric means of the MIC values at 48 h for all isolates tested were 0.05 and 0.5 μg/ml for RVC and FLC, respectively. Regimens of either RVC or FLC at 20 mg/kg twice daily were more effective to reduce the load of FLC-resistant C. albicans than single dose administration.
Complete eradication of C. albicans from the vagina was not observed with RVC or FLC treatment in the animal model, although RVC treatment showed a lower fungal concentration 14 days after drug administration.
外阴阴道念珠菌病是健康女性中常见的一种感染，主要由白色念珠菌引起，近年来其发病率显著上升。
本研究旨在比较雷夫康唑（RVC）和氟康唑（FLC）治疗实验性白色念珠菌性阴道炎的疗效。
对40株白色念珠菌进行体外对RVC和FLC敏感性的筛选。选择一株对FLC耐药（最低抑菌浓度[MIC]>64μg/ml）的白色念珠菌进行体内研究。小鼠阴道感染模型的治疗方案为：（1）1、5、10和20mg/kg RVC每日一次，（2）20mg/kg RVC每日两次，（3）20mg/kg FLC每日一次，（4）20mg/kg FLC每日两次。
所有受试菌株在48小时时MIC值的几何平均值，RVC和FLC分别为0.05和0.5μg/ml。20mg/kg RVC或FLC每日两次的治疗方案比单剂量给药更有效地降低了耐FLC白色念珠菌的负荷。
在动物模型中，RVC或FLC治疗均未观察到白色念珠菌从阴道完全清除，尽管RVC治疗在给药14天后显示出较低的真菌浓度。
