College of Marine Life Science, Ocean University of China, Qingdao 266003, China; Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China.
Fish Shellfish Immunol. 2013 Dec;35(6):1882-90. doi: 10.1016/j.fsi.2013.09.021. Epub 2013 Sep 29.
Extracellular signal-regulated kinase (ERK) is a serine/threonine-specific protein kinase, which participates in signaling transduction pathways that control intracellular events, including resumption of meiosis, embryogenesis, cell differentiation, cell proliferation, cell death and response to radiation. Some virus species evolved the ability to hijack the host cell ERK signaling transduction pathway for viral replications and gene expressions. To obtain a better understanding of ERK, we cloned a cDNA encoding ERK from the muscle of Fenneropenaeus chinensis (FcERK). The FcERK contained a 1098 bp open reading frame (ORF) encoding a protein of 365 amino acid residues with a conserved phosphorylation motif TEY in the kinase activation loop. Pair-wise and multiple sequence alignment revealed that ERK is highly conserved across taxa. The FcERK gene expressions in the hepatopancreas and gill were noticeably higher than the expression observed in the muscle. A challenge test was performed to reveal the responses of FcERK in different tissues to white spot syndrome virus (WSSV) infection. Post WSSV challenge, the FcERK expression in the gill significantly increased during the early stage of the viral infection, the FcERK expression in the muscle increased later than that in the gill, and the FcERK expression in the hepatopancreas significantly decreased. The FcERK gene expression profile accorded with the results that the virus primarily infects tissues originating from the ectoderm, with less infection of the tissues originating from the mesoderm, and hardly any infection in the tissues originating from the entoderm. Two single nucleotide polymorphisms (SNPs) were identified in the FcERK gene, involving C/T transition. The SNP genotypes of two groups of shrimps, respectively comprising 96 WSSV-resistant shrimps and 96 WSSV-susceptible shrimps were obtained using a high-resolution melting (HRM) method. In the two groups, the MAFs of both sites were greater than 0.05, and no site departed significantly (P < 0.05) from HWE. The genotype distributions of both mutation sites between the two groups were not significantly different. These results lead to a better understanding of the molecular mechanisms of the host-virus interaction and provide useful information for disease control.
细胞外信号调节激酶（ERK）是一种丝氨酸/苏氨酸特异性蛋白激酶，参与控制细胞内事件的信号转导途径，包括减数分裂恢复、胚胎发生、细胞分化、细胞增殖、细胞死亡和对辐射的反应。一些病毒物种进化出了劫持宿主细胞 ERK 信号转导途径的能力，以进行病毒复制和基因表达。为了更好地了解 ERK，我们从中国对虾（FcERK）肌肉中克隆了一个编码 ERK 的 cDNA。FcERK 包含一个 1098 bp 的开放阅读框（ORF），编码一个由 365 个氨基酸残基组成的蛋白质，在激酶激活环中有一个保守的磷酸化模体 TEY。两两和多重序列比对表明，ERK 在分类群中高度保守。在肝胰腺和鳃中，FcERK 的基因表达明显高于肌肉中的表达。进行了挑战试验以揭示不同组织中 FcERK 对白斑综合征病毒（WSSV）感染的反应。在 WSSV 感染后，病毒感染早期，鳃中 FcERK 的表达显著增加，肌肉中 FcERK 的表达晚于鳃中，肝胰腺中 FcERK 的表达显著下降。FcERK 基因表达谱与病毒主要感染外胚层起源的组织、较少感染中胚层起源的组织、几乎不感染内胚层起源的组织的结果一致。在 FcERK 基因中发现了两个单核苷酸多态性（SNP），涉及 C/T 转换。使用高分辨率熔解（HRM）方法获得了两组虾的 SNP 基因型，分别包括 96 只 WSSV 抗性虾和 96 只 WSSV 易感虾。在两组中，两个位点的 MAF 均大于 0.05，且没有一个位点显著偏离（P < 0.05）哈温平衡。两个突变位点的基因型在两组之间没有显著差异。这些结果有助于更好地了解宿主-病毒相互作用的分子机制，并为疾病控制提供有用信息。
