Bach R G, Meruelo D
Virology. 1985 Aug;145(1):165-70. doi: 10.1016/0042-6822(85)90211-9.
The p15(E)-related polypeptides of radiation leukemia virus (RadLV)-derived viruses and of cells infected with prototype MuLV were analyzed by immunoprecipitation, SDS-PAGE, and immunofluorescence analyses. It was found that the p15(E)-related molecules of ecotropic and xenotropic viruses derived from RadLV lymphoma cell lines were distinguishable by reactivity with monoclonal anti-p15(E) antibodies and by SDS-PAGE profile. Ecotropic MuLV of RadLV origin encoded the p15(E)a antigen and produced a Pr15(E) of 20K MW. In contrast, xenotropic virus derived from RadLV did not express the p15(E)a antigen and by SDS-PAGE its Pr15(E) migrated at 21K. A previously undescribed, p15(E)-related molecule of 18.5K MW was associated with xenotropic RadLV. These differences were also reproduced by the prototype ecotropic, xenotropic, and dualtropic viruses.
通过免疫沉淀、SDS - PAGE和免疫荧光分析，对辐射白血病病毒（RadLV）衍生病毒以及感染原型鼠白血病病毒（MuLV）的细胞中的p15（E）相关多肽进行了分析。结果发现，源自RadLV淋巴瘤细胞系的亲嗜性和异嗜性病毒的p15（E）相关分子，可通过与单克隆抗p15（E）抗体的反应性以及SDS - PAGE图谱加以区分。源自RadLV的亲嗜性MuLV编码p15（E）a抗原，并产生分子量为20K的Pr15（E）。相比之下，源自RadLV的异嗜性病毒不表达p15（E）a抗原，其Pr15（E）在SDS - PAGE中迁移至21K处。一种先前未描述的分子量为18.5K的p15（E）相关分子与异嗜性RadLV有关。原型亲嗜性、异嗜性和双嗜性病毒也呈现出这些差异。
