[利用重组人β-防御素-3评估白色念珠菌在软衬材料表面的黏附作用]
[Use of recombinant human beta-defensin-3 to evaluate the effect of adhesion of Candida albicans on the surface of soft lining material].
作者信息
Shi Yong, Song Wei, Liu Xiao-fang, Gao Li-min, Song Guang-bao
机构信息
.Department of Prosthodontics, Guangdong Provincial Stomatological Hospital, Affiliated Hospital of Southern Medical University. Guangzhou 510280;China.E-mail:
出版信息
Shanghai Kou Qiang Yi Xue. 2013 Aug;22(4):389-92.
PURPOSE
To evaluate the effectiveness of recombinant human beta-defensin-3 (rHBD3) on reduction of Candida albicans from the surface of soft lining materials.
METHODS
Specimens made of soft lining materials, which had been contaminated with C. albicans, were immersed in 25 μg/mL rHBD3 and 5.25% sodium hypochlorite (a positive control) for 5, 10, 30 and 60 min. The results were analyzed statistically with SPSS 10.0 software package. Confocal laser scanning microscopy(CLSM) of biofilm-associated C. albicans were treated with rHBD3 in different times.
RESULTS
The results confirmed that an immersion time of 30 min or higher yielded a good disinfection effect, both for the experimental group and the positive control. A large number of pathogenic bacteria were killed and the cells were stained red by SYTO9/PI after 30 min.
CONCLUSIONS
It is possible that soft lining materials immersed in rHBD3 solution that possesses antifungal activity may be clinically protective.
目的
评估重组人β-防御素-3（rHBD3）对减少软衬材料表面白色念珠菌的有效性。
方法
将已被白色念珠菌污染的软衬材料制成的标本分别浸入25μg/mL的rHBD3和5.25%次氯酸钠（阳性对照）中5、10、30和60分钟。使用SPSS 10.0软件包对结果进行统计学分析。对生物膜相关白色念珠菌用不同时间的rHBD3进行共聚焦激光扫描显微镜（CLSM）观察。
结果
结果证实，浸泡30分钟及以上时间，实验组和阳性对照均产生了良好的消毒效果。30分钟后大量病原菌被杀死，细胞被SYTO9/PI染成红色。
结论
浸泡在具有抗真菌活性的rHBD3溶液中的软衬材料在临床上可能具有保护作用。
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