Contribution of restricted extracellular space to the inactivation of calcium current in the snail neuron.

The mechanisms underlying the inactivation of calcium current (ICa) were investigated in isolated nerve cell bodies of Helix aspersa using a suction pipette technique that allowed voltage clamp and internal perfusion at the same time. ICa was recorded after eliminating the Na and K currents by removing Na+ and K+ both in external and internal solutions, and ICa inactivation due to intracellular Ca2+ accumulation was blocked by 5-25 mM EGTA. The inactivation rates of ICa, IBa and ISr corresponded to two exponential processes. The inactivation rates of the inward currents (IMn, ICd and IZn) less than 1/5 of ICa fitted a single exponential. However, when neurons were superfused with hypertonic external solution by adding 100 mM sucrose together with internal EGTA, the steady-state inactivation of ICa, IBa and ISr was reduced, and the inactivation processes changed to a single exponential similar to that of IMn, ICd and IZn. In contrast, internal perfusion with the hypertonic solution had no effect on the inactivation of ICa, IBa and ISr. Therefore, it was concluded that the inactivation process of ICa is dependent not only on the membrane voltage and the intracellular Ca2+ accumulation as described previously, but is also affected by the rapid fall in the concentration of Ca2+ in the restricted extracellular spaces (RES) which gets enlarged by the hypertonic external solution. The same is also true for IBa and ISr.