高容量腺病毒载体的生产。
Production of high-capacity adenovirus vectors.
作者信息
Kreppel Florian
机构信息
Department of Gene Therapy, University of Ulm, Ulm, Germany.
出版信息
Methods Mol Biol. 2014;1089:211-29. doi: 10.1007/978-1-62703-679-5_15.
High-capacity adenoviral vectors (HC-Ad), also known as "helper-dependent" (HD-Ad), "gutless", "gutted", or "third-generation" Ad vectors, are devoid of all viral coding sequences and have shown promising potential for a wide variety of different applications-from classic gene therapy to genetic vaccination and tumor treatment. However, compared to first-generation adenoviral vectors their production is more complex and requires specific in-depth knowledge. This chapter delivers a detailed protocol for the successful production of HC-Ad vectors to high titers.
高容量腺病毒载体（HC-Ad），也被称为“辅助依赖型”（HD-Ad）、“无基因组型”、“基因组缺失型”或“第三代”腺病毒载体，不含所有病毒编码序列，并且在从经典基因治疗到基因疫苗接种和肿瘤治疗等各种各样的不同应用中显示出了有前景的潜力。然而，与第一代腺病毒载体相比，其生产更为复杂，需要特定的深入知识。本章提供了一个成功生产高滴度HC-Ad载体的详细方案。
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