Departments of Microbiology and Clinical Microbiology, Faculty of Medicine, Gaziosmanpaşa University, Tokat, Turkey.
Int J Dermatol. 2013 Dec;52(12):1557-60. doi: 10.1111/ijd.12100. Epub 2013 Oct 18.
In this study, we aimed to establish the in vitro antifungal susceptibilities of terbinafine, miconazole, itraconazole, ketoconazole, griseofulvin, and amphotericin B against dermatophyte isolates.
One hundred and seventy-seven clinical isolates were tested: Trichophyton rubrum (n = 78), Trichophyton mentagrophytes (n = 49), Epidermophyton floccosum (n = 30), Trichophyton verrucosum (n = 16), and Trichophyton tonsurans (n = 4). The broth microdilution assay for antifungal susceptibility testing of dermatophytes was performed according to Clinical and Laboratory Standards Institute guidelines in the M38-A2 document.
Our minimum inhibitory concentration (MIC) results showed that the values for terbinafine for all dermatophyte isolates were significantly lower than the values for amphotericin B, miconazole, itraconazole, ketoconazole, and griseofulvin. For T. rubrum isolates, amphotericin B was more active than miconazole, itraconazole, and ketoconazole. Among the antifungal drugs tested, griseofulvin had the highest minimum inhibitory concentration values for T. mentagrophytes isolates.
Terbinafine was found to be the most effective antifungal drug against all tested dermatophyte isolates. Griseofulvin was the less active antifungal drug against T. mentagrophytes isolates. Performing antifungal susceptibility testing is especially important for screening the development of antifungal resistance.
本研究旨在建立特比萘芬、咪康唑、伊曲康唑、酮康唑、灰黄霉素和两性霉素 B 对皮肤癣菌分离株的体外抗真菌药敏性。
共检测了 177 株临床分离株：红色毛癣菌（n=78）、须癣毛癣菌（n=49）、絮状表皮癣菌（n=30）、疣状毛癣菌（n=16）和断发毛癣菌（n=4）。根据临床和实验室标准协会在 M38-A2 文档中的指南，进行皮肤癣菌抗真菌药敏试验的肉汤微量稀释法。
我们的最小抑菌浓度（MIC）结果表明，特比萘芬对所有皮肤癣菌分离株的数值均显著低于两性霉素 B、咪康唑、伊曲康唑、酮康唑和灰黄霉素。对于红色毛癣菌分离株，两性霉素 B 比咪康唑、伊曲康唑和酮康唑更有效。在测试的抗真菌药物中，灰黄霉素对须癣毛癣菌分离株的最低抑菌浓度值最高。
特比萘芬被发现是所有测试的皮肤癣菌分离株最有效的抗真菌药物。灰黄霉素是对须癣毛癣菌分离株活性最低的抗真菌药物。进行抗真菌药敏试验对于筛选抗真菌药物耐药性的发展尤为重要。
