胚胎肝脏中脂联寡糖的生物合成。含甘露糖衍生物的形成。

Biosynthesis of lipid-linked oligosaccharides in embryonic liver. Formation of mannose containing derivatives.

作者信息

Kyosseva S V, Zhivkov V I

出版信息

Int J Biochem. 1985;17(7):813-7. doi: 10.1016/0020-711x(85)90269-1.

Abstract

In the presence of exogenous dolichyl phosphate mannosyl transferase activity towards dolichyl phosphate was nearly 3-fold higher in microsomes from pig embryonic liver compared to that from adult liver. After incubation of microsomes from embryonic liver with UDP-N-acetylglucosamine and GDP-[14C]mannose lipid-linked tri- to undecasaccharides were discovered in CHCl3-CH3OH (2:1, v/v) and CHCl3-CH3OH-H2O (1:1:0.3, by vol) extracts. The main proportion of the radioactivity was incorporated into penta-, sexta and undecasaccharides. Amphomycin at concentration 500 micrograms/ml inhibited almost completely dolichyl phosphate mannose synthesis in embryonic liver microsomes without inhibition the formation of lipid-linked penta- and sextasaccharides. It was suggested that mannose transferred to lipid-linked tetra- to heptasaccharides comes from GDP-mannose but not from dolichyl phosphate mannose.

摘要

在外源多萜醇磷酸甘露糖基转移酶存在的情况下，猪胚胎肝脏微粒体中针对多萜醇磷酸的活性比成年肝脏微粒体中的活性高近3倍。用UDP-N-乙酰葡糖胺孵育胚胎肝脏微粒体后，在CHCl3-CH3OH（2:1，v/v）和CHCl3-CH3OH-H2O（1:1:0.3，体积比）提取物中发现了脂质连接的三糖至十一糖。放射性的主要部分掺入了五糖、六糖和十一糖中。浓度为500微克/毫升的两性霉素几乎完全抑制了胚胎肝脏微粒体中多萜醇磷酸甘露糖的合成，但不抑制脂质连接的五糖和六糖的形成。有人提出，转移到脂质连接的四糖至七糖上的甘露糖来自GDP-甘露糖，而不是来自多萜醇磷酸甘露糖。