Zeng Jijiao, Helms Gregory L, Gao Xin, Chen Shulin
Department of Biological Systems Engineering, The Bioprocessing and Bioproduct Engineering Laboratory (BBEL), L. J. Smith 213, Washington State University , Pullman, Washington 99163 United States.
J Agric Food Chem. 2013 Nov 20;61(46):10848-57. doi: 10.1021/jf4030486. Epub 2013 Nov 5.
A further understanding of the structure of lignin from herbaceous crops is needed for advancing technologies of lignocellulosic biomass processing and utilization. A method was established in this study for analyzing structural motifs found in milled straw lignin (MSL) and cellulase-digested lignin (CEL) isolated from wheat straw by combining quantitative (13)C and HSQC NMR spectral analyses. The results showed that guaiacyl (G) was the predominant unit in wheat straw cell wall lignin over syringyl (S) and hydroxyphenyl (H) units. Up to 8.0 units of tricin were also detected in wheat straw lignin per 100 aromatic rings. Various interunit linkages, including β-O-4, β-5, β-β', β-1, α, β-diaryl ether, and 5-5'/4-O-β' as well as potential lignin-carbohydrate complex (LCC) bonds, were identified and quantified. These findings provide useful information for the development of biofuels and lignin-based materials.
为推动木质纤维素生物质加工与利用技术的发展，需要进一步了解草本作物中木质素的结构。本研究建立了一种方法，通过结合定量(13)C和HSQC NMR光谱分析，来分析从小麦秸秆中分离出的磨碎秸秆木质素(MSL)和纤维素酶消化木质素(CEL)中的结构基序。结果表明，在小麦秸秆细胞壁木质素中，愈创木基(G)单元比紫丁香基(S)和对羟基苯基(H)单元更占主导地位。每100个芳香环中，小麦秸秆木质素中还检测到多达8.0个三嗪单元。鉴定并定量了各种单元间连接，包括β-O-4、β-5、β-β'、β-1、α、β-二芳基醚和5-5'/4-O-β'以及潜在的木质素-碳水化合物复合物(LCC)键。这些发现为生物燃料和木质素基材料的开发提供了有用信息。
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