表皮生长因子受体 2（ERBB2）表达对脑胶质瘤 TJ905 细胞侵袭性的影响。
Effect of ERBB2 expression on invasiveness of glioma TJ905 cells.
机构信息
Department of Neurosurgery, First Hospital of Xi'an, Jiaotong University, Xi'an 710061, China.
出版信息
Asian Pac J Trop Med. 2013 Dec;6(12):964-7. doi: 10.1016/S1995-7645(13)60172-8.
OBJECTIVE
To investigate the influence and possible mechanism of ERBB2 expression on the invasiveness of glioma cells.
METHODS
Glioma TJ905 cells were separated and cultured. ERBB2 shRNA and overexpressing vectors were constructed, which were then transfected. The ERBB2 expression was up-regulated or down-regulated. Changes of invasiveness of TJ905 cells were detected by Transwell assay, and the expressions of matrix metalloprotease (MMP)-2 and MMP-9 were measured by Western blot.
RESULTS
ERBB2 shRNA transfection vector could effectively inhibit expression of ERBB2; while ERBB2 overexpressing vector transfection could significantly improve the expression of ERBB2 in TJ905 cells. Transwell assay showed that when ERBB2 expression was down-regulated, the invasiveness of TJ905 cells was notably decreased; when ERBB2 expression was up-regulated, the invasiveness of TJ905 cells was markedly increased. Meanwhile, Western blot indicated that down-regulating ERBB2 inhibited the expression of MMP-2 and MMP-9, while up-regulating ERBB2 enhanced their expressions.
CONCLUSIONS
ERBB2 expression is closely related to the invasiveness of glioma TJ905 cells.
目的
探讨 ERBB2 表达对脑胶质瘤细胞侵袭性的影响及其可能的机制。
方法
分离培养脑胶质瘤 TJ905 细胞，构建 ERBB2 shRNA 和过表达载体，转染后上调或下调 ERBB2 表达，采用 Transwell 小室侵袭实验检测 TJ905 细胞侵袭能力的变化，Western blot 检测细胞基质金属蛋白酶（MMP）-2、MMP-9 的表达。
结果
ERBB2 shRNA 转染载体能有效抑制 ERBB2 的表达，而 ERBB2 过表达载体转染能显著提高 TJ905 细胞 ERBB2 的表达。Transwell 小室侵袭实验显示，下调 ERBB2 表达后 TJ905 细胞的侵袭能力明显下降，而上调 ERBB2 表达后 TJ905 细胞的侵袭能力显著增强。同时，Western blot 结果表明下调 ERBB2 抑制了 MMP-2 和 MMP-9 的表达，而上调 ERBB2 则增强了它们的表达。
结论
ERBB2 表达与脑胶质瘤 TJ905 细胞的侵袭性密切相关。
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