• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

胰腺β细胞中的电压门控性Ca2+电流

Voltage-gated Ca2+ current in pancreatic B-cells.

作者信息

Satin L S, Cook D L

出版信息

Pflugers Arch. 1985 Aug;404(4):385-7. doi: 10.1007/BF00585354.

DOI:10.1007/BF00585354
PMID:2414720
Abstract

Voltage-dependent inward Ba++ and Ca++ currents were recorded in cultured neonatal rat pancreatic islet cells using the whole-cell voltage clamp technique. Outward current was suppressed by internal Cs+ and ATP and external TEA. Inward currents activated rapidly and decayed to a variable extent. The current decay was particularly marked when using long duration or large depolarizing pulses. Currents were due to Ca++ channel activation since they were abolished by omitting Ba++ and Ca++ or including Co++.

摘要

采用全细胞电压钳技术,在培养的新生大鼠胰岛细胞中记录到电压依赖性内向钡离子(Ba++)和钙离子(Ca++)电流。内向电流被细胞内的铯离子(Cs+)、三磷酸腺苷(ATP)以及细胞外的四乙铵(TEA)抑制。内向电流迅速激活,并在不同程度上衰减。当使用持续时间长或去极化幅度大的脉冲时,电流衰减尤为明显。这些电流是由钙离子通道激活引起的,因为当去除钡离子和钙离子或加入钴离子时,电流消失。

相似文献

1
Voltage-gated Ca2+ current in pancreatic B-cells.胰腺β细胞中的电压门控性Ca2+电流
Pflugers Arch. 1985 Aug;404(4):385-7. doi: 10.1007/BF00585354.
2
Calcium and delayed potassium currents in mouse pancreatic beta-cells under voltage-clamp conditions.电压钳条件下小鼠胰腺β细胞中的钙电流和延迟钾电流。
J Physiol. 1986 May;374:531-50. doi: 10.1113/jphysiol.1986.sp016096.
3
A calcium-activated chloride current generates the after-depolarization of rat sensory neurones in culture.一种钙激活氯离子电流产生培养的大鼠感觉神经元的去极化后电位。
J Physiol. 1985 Jul;364:217-39. doi: 10.1113/jphysiol.1985.sp015740.
4
A calcium-dependent chloride current in insulin-secreting beta TC-3 cells.胰岛素分泌β-TC-3细胞中的一种钙依赖性氯电流。
Pflugers Arch. 1997 Apr;433(6):679-90. doi: 10.1007/s004240050332.
5
The calcium channel current of pregnant rat single myometrial cells in short-term primary culture.短期原代培养的孕鼠单个子宫肌层细胞的钙通道电流
J Physiol. 1987 Nov;392:253-72. doi: 10.1113/jphysiol.1987.sp016779.
6
Patch-clamp study of the calcium-dependent chloride current in AtT-20 pituitary cells.AtT-20垂体细胞中钙依赖性氯电流的膜片钳研究
J Neurophysiol. 1987 Dec;58(6):1431-51. doi: 10.1152/jn.1987.58.6.1431.
7
Inactivation of calcium channels in mammalian heart cells: joint dependence on membrane potential and intracellular calcium.哺乳动物心脏细胞中钙通道的失活:对膜电位和细胞内钙的联合依赖性。
J Physiol. 1985 Jul;364:395-411. doi: 10.1113/jphysiol.1985.sp015752.
8
Voltage-activated calcium and potassium currents in human pancreatic beta-cells.人类胰腺β细胞中的电压激活钙电流和钾电流。
J Physiol. 1991 Nov;443:175-92. doi: 10.1113/jphysiol.1991.sp018829.
9
Voltage-activated membrane currents in rat cerebellar granule neurones.大鼠小脑颗粒神经元中的电压激活膜电流。
J Physiol. 1989 Jul;414:179-99. doi: 10.1113/jphysiol.1989.sp017683.
10
Voltage-gated Ca current in pancreatic islet beta-cells.胰岛β细胞中的电压门控钙电流
Adv Exp Med Biol. 1986;211:189-93. doi: 10.1007/978-1-4684-5314-0_16.

引用本文的文献

1
VDAC1 is a target for pharmacologically induced insulin hypersecretion in β cells.电压依赖性阴离子通道1(VDAC1)是β细胞中药物诱导胰岛素分泌过多的一个靶点。
Cell Rep. 2025 Jun 24;44(6):115834. doi: 10.1016/j.celrep.2025.115834. Epub 2025 Jun 11.
2
DOC2b Enhances β-Cell Function via a Novel Tyrosine Phosphorylation-Dependent Mechanism.DOC2b 通过一种新型酪氨酸磷酸化依赖的机制增强β细胞功能。
Diabetes. 2022 Jun 1;71(6):1246-1260. doi: 10.2337/db21-0681.
3
Nutrient Regulation of Pancreatic Islet β-Cell Secretory Capacity and Insulin Production.

本文引用的文献

1
Calcium action potentials and potassium permeability activation in pancreatic beta-cells.胰腺β细胞中的钙动作电位和钾通透性激活
Am J Physiol. 1980 Sep;239(3):C124-33. doi: 10.1152/ajpcell.1980.239.3.C124.
2
Plateau potentials in pancreatic islet cells are voltage-dependent action potentials.胰岛细胞中的平台电位是电压依赖性动作电位。
Nature. 1980 Jul 24;286(5771):404-6. doi: 10.1038/286404a0.
3
Pancreatic A cell generates action potential.胰腺A细胞产生动作电位。
营养素对胰岛β细胞分泌能力和胰岛素生成的调节作用。
Biomolecules. 2022 Feb 20;12(2):335. doi: 10.3390/biom12020335.
4
Beta-Cell Ion Channels and Their Role in Regulating Insulin Secretion.β 细胞离子通道及其在胰岛素分泌调节中的作用。
Compr Physiol. 2021 Oct 12;11(4):1-21. doi: 10.1002/cphy.c210004.
5
Intercellular Communication in the Islet of Langerhans in Health and Disease.胰岛内细胞间通讯在健康和疾病中的作用。
Compr Physiol. 2021 Jun 30;11(3):2191-2225. doi: 10.1002/cphy.c200026.
6
Mitochondrial Efflux of Citrate and Isocitrate Is Fully Dispensable for Glucose-Stimulated Insulin Secretion and Pancreatic Islet β-Cell Function.柠檬酸和异柠檬酸的线粒体外排对于葡萄糖刺激的胰岛素分泌和胰岛β细胞功能是完全可有可无的。
Diabetes. 2021 Aug;70(8):1717-1728. doi: 10.2337/db21-0037. Epub 2021 May 26.
7
Conventional and Unconventional Mechanisms by which Exocytosis Proteins Oversee β-cell Function and Protection.外排蛋白调控β细胞功能和保护的传统和非传统机制。
Int J Mol Sci. 2021 Feb 12;22(4):1833. doi: 10.3390/ijms22041833.
8
Overexpression of Gjb4 impairs cell proliferation and insulin secretion in primary islet cells.Gjb4 的过表达会损害原代胰岛细胞的增殖和胰岛素分泌。
Mol Metab. 2020 Nov;41:101042. doi: 10.1016/j.molmet.2020.101042. Epub 2020 Jun 18.
9
Recent Insights into Beta-cell Exocytosis in Type 2 Diabetes.2 型糖尿病中β细胞胞吐作用的最新见解。
J Mol Biol. 2020 Mar 6;432(5):1310-1325. doi: 10.1016/j.jmb.2019.12.012. Epub 2019 Dec 19.
10
Mechanisms of the amplifying pathway of insulin secretion in the β cell.β 细胞胰岛素分泌的放大途径的机制。
Pharmacol Ther. 2017 Nov;179:17-30. doi: 10.1016/j.pharmthera.2017.05.003. Epub 2017 May 18.
Jpn J Physiol. 1982;32(5):873-8. doi: 10.2170/jjphysiol.32.873.
4
Phasic glucose-stimulated insulin secretion by neonatal rat pancreatic islet cells. Enhancement by sodium salicylate.新生大鼠胰岛细胞的阶段性葡萄糖刺激胰岛素分泌。水杨酸钠的增强作用。
Diabetes. 1984 Sep;33(9):872-8. doi: 10.2337/diab.33.9.872.
5
Electrical pacemaker mechanisms of pancreatic islet cells.胰岛细胞的电起搏机制。
Fed Proc. 1984 Jun;43(9):2368-72.
6
Sodium and calcium channels in bovine chromaffin cells.牛嗜铬细胞中的钠通道和钙通道。
J Physiol. 1982 Oct;331:599-635. doi: 10.1113/jphysiol.1982.sp014394.
7
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.用于从细胞和无细胞膜片进行高分辨率电流记录的改进膜片钳技术。
Pflugers Arch. 1981 Aug;391(2):85-100. doi: 10.1007/BF00656997.
8
Calcium channel.钙通道
Annu Rev Neurosci. 1981;4:69-125. doi: 10.1146/annurev.ne.04.030181.000441.
9
Regulation of insulin release by calcium.钙对胰岛素释放的调节。
Physiol Rev. 1981 Oct;61(4):914-73. doi: 10.1152/physrev.1981.61.4.914.
10
Intracellular ATP directly blocks K+ channels in pancreatic B-cells.细胞内的三磷酸腺苷(ATP)直接阻断胰腺β细胞中的钾离子通道。
Nature. 1984;311(5983):271-3. doi: 10.1038/311271a0.