Palani Gunasekaran, Padmanabhan Padma Priya, Ramesh Kiruba, Asadullah Khaleefathullah Sheriff, Sambasivam Mohana, Arunagiri Kavita, Krishnasamy Kaveri
Department of Virology, King Institute of Preventive Medicine and Research, Guindy, Chennai, Tamil Nadu, India.
Indian J Pathol Microbiol. 2013 Jul-Sep;56(3):269-71. doi: 10.4103/0377-4929.120391.
In this study, we report the evaluation of In-house flavi virus immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA), which can be used as a screening test to determine the infecting flavivirus serotype over the current serological methods. A panel of 88 sera (inclusive of well characterized dengue, Japanese Encephalitis (JE) and West Nile virus (WNV) positive and negative samples tested and confirmed by commercial kit) was used for evaluation of the kit. The sensitivity and specificity of the In-house capture assay versus the commercial kit for the sero-diagnosis of dengue was 100% and 87% respectively, for JE IgM, it was found to be 90% and 100% respectively, and for West Nile it was 87.5% and 90.9%. Based on the study, we concluded that this flavivirus-serotyping ELISA provides rapid results and may be used as an accurate alternate to other serological tests for the specific diagnosis of flavivirus infections.
在本研究中，我们报告了对内部黄病毒免疫球蛋白M（IgM）捕获酶联免疫吸附测定（ELISA）的评估，该测定可作为一种筛查试验，用于确定感染的黄病毒血清型，优于当前的血清学方法。使用一组88份血清（包括经商业试剂盒检测和确认的特征明确的登革热、日本脑炎（JE）和西尼罗河病毒（WNV）阳性和阴性样本）对该试剂盒进行评估。内部捕获测定法与商业试剂盒相比，登革热血清诊断的敏感性和特异性分别为100%和87%，JE IgM的敏感性和特异性分别为90%和100%，西尼罗河病毒的敏感性和特异性分别为87.5%和90.9%。基于该研究，我们得出结论，这种黄病毒血清分型ELISA能提供快速结果，可作为其他血清学检测的准确替代方法，用于黄病毒感染的特异性诊断。
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