得力生注射液（一种中药复方制剂）增强了顺铂对肺癌细胞系PGCL3的作用。

Delisheng Injection (), a Chinese medicinal compound, enhanced the effect of cis-platinum on lung carcinoma cell line PGCL3.

作者信息

Dong Xi-lin, Gong Ying, Chen Zhen-zhen, Wang Ya-juan

机构信息

Department of Respiratory, the Second Affiliated Hospital, College of Medicine of Xi'an Jiaotong University, Xi'an, 710004, China.

Department of Pharmacy, the Fourth People's, Hospital of Ji'nan City, Ji'nan, 250031, China.

出版信息

Chin J Integr Med. 2014 Apr;20(4):286-91. doi: 10.1007/s11655-013-1335-0. Epub 2013 Oct 23.

DOI:10.1007/s11655-013-1335-0

PMID:24155071

Abstract

OBJECTIVE

To investigate the effect of Delisheng Injection (, DLS), a Chinese medicinal compound, DLS combined with cis-platinum (DDP), an active agent used in lung cancer chemotherapy, on a human highly metastatic giant lung carcinoma cell line PGCL3.

METHODS

The suspended PGCL3 cells at 10(5) /mL cultured in 96-well tissue culture plates were divided into 4 groups: DLS treatment group (2 μL/mL, 5 μL/mL, 10 μL/mL, 25 μL/mL), DDP treatment group (1 μg/mL, 2 μg/mL, 5 μg/mL, 15 μg/mL), combined DLS with DDP treatment group (DLS:DDP 2 μL/mL:1 μg/mL, 5 μL/mL:2 μg/mL, 10 μL/mL:5 μg/mL, 25 μL/mL:15 μg/mL) and a control group. The cytotoxicity of DLS with different concentrations (2 μL/mL, 5 μL/mL, 10 μL/mL, 25 μL/mL) on PGCL3 cells was determined by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay. Effect of DLS on adhesion of PGCL-3 cells was tested by cell-matrigel adhesion assay. Chemotactic movement model of transwell camerula was used to determine the effect of DLS on invasion and migration of PGCL-3 cells.

RESULTS

Compared with the control group, DLS (2 μL/mL, 5 μL/mL, 10 μL/mL, 25 μL/mL) could significantly decrease cell proliferation, adhesion, invasion and migration abilities (P <0.05). Cell adhesion, invasion and migration abilities were significantly decreased after combination treatment of DLS:DDP (2 μL/mL:1 μg/mL, 5 μL/mL:2 μg/mL, 10 μL/mL:5 μg/mL, 25 μL/mL:15 μg/mL) compared with DDP single-agent treatment (1 μg/mL, 2 μg/mL, 5 μg/mL, 15 μg/mL, P<0.05), respectively.

CONCLUSIONS

DLS single-agent has a satisfying inhibition effect in PGCL3 cell line and DLS might enhance the inhibition effect of DDP on cancer metastasis. Our research provided a experimental basis about the treatment on highly metastatic lung caner.

摘要

目的

研究中药复方得力生注射液（DLS）以及DLS联合肺癌化疗常用药顺铂（DDP）对人高转移巨细胞肺癌细胞系PGCL3的作用。

方法

将培养于96孔板中密度为10(5)/mL的PGCL3悬浮细胞分为4组：DLS处理组（2 μL/mL、5 μL/mL、10 μL/mL、25 μL/mL）、DDP处理组（1 μg/mL、2 μg/mL、5 μg/mL、15 μg/mL）、DLS与DDP联合处理组（DLS:DDP为2 μL/mL:1 μg/mL、5 μL/mL:2 μg/mL、10 μL/mL:5 μg/mL、25 μL/mL:15 μg/mL）和对照组。采用噻唑蓝（MTT）法检测不同浓度（2 μL/mL、5 μL/mL、10 μL/mL、25 μL/mL）DLS对PGCL3细胞的细胞毒性。通过细胞-基质胶黏附实验检测DLS对PGCL-3细胞黏附的影响。采用Transwell小室趋化运动模型检测DLS对PGCL-3细胞侵袭和迁移的影响。

结果

与对照组相比，DLS（2 μL/mL、5 μL/mL、10 μL/mL、25 μL/mL）可显著降低细胞增殖、黏附、侵袭和迁移能力（P<0.05）。与DDP单药处理组（1 μg/mL、2 μg/mL、5 μg/mL、15 μg/mL）相比，DLS:DDP联合处理组（2 μL/mL:1 μg/mL、5 μL/mL:2 μg/mL、10 μL/mL:5 μg/mL、25 μL/mL:15 μg/mL）细胞黏附及侵袭和迁移能力均显著降低（P<0.05）。