Moroki Takayasu, Yoshikawa Yutaka, Yoshizawa Katsuhiko, Tsubura Airo, Yasui Hiroyuki
Department of Analytical and Bioinorganic Chemistry, Division of Analytical and Physical Chemistry, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
J Toxicol Pathol. 2013 Sep;26(3):329-33. doi: 10.1293/tox.26.329. Epub 2013 Oct 15.
Vanadium has potential for use in diabetes therapy. Many investigators have reported toxic effects of inorganic vanadium salts; however, there are few reports on toxic effects of oxovanadium(VO(2+)) complexes. Therefore, we studied VO(2+) toxicity by examining histological changes and measuring the vanadium concentration in the testis after repeated oral administration of bis(1-oxy-2-pyridine-thiolato)oxovanadium(VO(2+)) (VO(opt)2) for 2 or 4 weeks in KK-A(y) mice. Severe mineralization and degeneration/necrosis of the seminiferous tubules were detected after either 2 or 4 weeks of administration. Vacuolar changes in Sertoli cells and the seminiferous epithelia, and hyperplasia of Leydig cells were observed in the testes of some animals. Vanadium concentrations in the mineralized testis were much higher than those in the testis of untreated KK-A(y) mice. These results represent the first report of the possibility for seminiferous tubules mineralization induced by VO(opt)2 administration. Therefore, our research provides important information about the potentially toxic effects of VO(2+) complexes.
钒在糖尿病治疗方面具有应用潜力。许多研究者报告了无机钒盐的毒性作用；然而，关于氧钒（VO(2+)）配合物毒性作用的报道却很少。因此，我们通过在KK-A(y)小鼠中连续2周或4周口服双（1-氧代-2-吡啶硫醇根）氧钒（VO(2+)）（VO(opt)2），检查组织学变化并测量睾丸中的钒浓度，来研究VO(2+)的毒性。给药2周或4周后均检测到生精小管严重矿化和变性/坏死。在一些动物的睾丸中观察到支持细胞和生精上皮的空泡样变化以及间质细胞增生。矿化睾丸中的钒浓度远高于未处理的KK-A(y)小鼠睾丸中的钒浓度。这些结果首次报道了VO(opt)2给药诱导生精小管矿化的可能性。因此，我们的研究提供了关于VO(2+)配合物潜在毒性作用的重要信息。
