Chang Y S, Papkoff H
Gen Comp Endocrinol. 1985 Dec;60(3):372-9. doi: 10.1016/0016-6480(85)90070-x.
Sea turtle prolactin (PRL) was isolated in a highly purified state from sea turtle pituitary side fractions obtained from other studies and some of its biological, chemical, and immunological properties were determined. Sea turtle PRL is a protein of 22-24 kDa [sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis]. Its sole amino terminal amino acid residue is leucine. The amino acid composition of sea turtle PRL is similar to ovine PRL and is characterized by a high content of aspartic acid (20 residues), glutamic acid (34 residues), serine (19 residues), and leucine (24 residues). It possess three disulfide bonds and 2 tryptophan residues which is also characteristic of many species of PRL. As with PRLs of other species, it displayed multiple stained bands in disc gel electrophoresis, at pH 8.3. Biologically, sea turtle PRL was active in pigeon crop-sac assay but the dose-response characteristics were nonparallel when compared to ovine PRL. An antiserum against sea turtle PRL was raised in rabbit and a homologous radioimmunoassay was developed with a sensitivity of 2.8 ng for sea turtle PRL. Sea turtle gonadotropins did not cross-react, but sea turtle growth hormone showed a 5% cross-reactivity. Pituitary extracts from other species of turtles displayed parallel inhibition curves to the sea turtle PRL. Extracts and prolactin preparations from several birds, snakes, alligator, and marsupials cross-reacted, but in a nonparallel fashion. Bullfrog pituitary extract and Tilapia PRL showed no cross-reaction at high doses. Several purified mammalian PRLs (pig, sheep, human, horse, dog) showed minimal or no ability to cross-react in the RIA.
从其他研究中获得的海龟垂体侧部分中以高纯度状态分离出海龟催乳素（PRL），并确定了其一些生物学、化学和免疫学特性。海龟PRL是一种22 - 24 kDa的蛋白质[十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳]。其唯一的氨基末端氨基酸残基是亮氨酸。海龟PRL的氨基酸组成与绵羊PRL相似，其特点是天冬氨酸（20个残基）、谷氨酸（34个残基）、丝氨酸（19个残基）和亮氨酸（24个残基）含量高。它有三个二硫键和2个色氨酸残基，这也是许多PRL物种的特征。与其他物种的PRL一样，在pH 8.3的圆盘凝胶电泳中它显示出多条染色带。在生物学上，海龟PRL在鸽嗉囊试验中具有活性，但与绵羊PRL相比，剂量反应特征不平行。用兔制备了抗海龟PRL的抗血清，并开发了一种同源放射免疫测定法，对海龟PRL的灵敏度为2.8 ng。海龟促性腺激素没有交叉反应，但海龟生长激素显示出5%的交叉反应性。其他海龟物种的垂体提取物对海龟PRL显示出平行的抑制曲线。几种鸟类、蛇类、短吻鳄和有袋动物的提取物及催乳素制剂有交叉反应，但方式不平行。牛蛙垂体提取物和罗非鱼PRL在高剂量时没有交叉反应。几种纯化的哺乳动物PRL（猪、绵羊、人、马、狗）在放射免疫测定中显示出最小的交叉反应能力或无交叉反应能力。
