Institute of Microbiology, University of Bern, CH-3013, Bern, Switzerland.
Curr Genet. 1984 Feb;8(2):99-105. doi: 10.1007/BF00420225.
Purines such as hypoxanthine, xanthine, uric acid, allantoin and allantoic acid serve as sole nitrogen sources for the yeast Schizosaccharomyces pombe. A number of classes of mutants unable to use purines have been isolated and genetically analysed. Mutants in the urol gene lack uricase, all1 lack allantoinase, ala1 lack allantoicase whilst in ure1, ure2, ure3 and ure4 genes lack urease activity. Mutants in four hyp genes are unable to convert hypoxanthine to uric acid whilst mutation in xan1 results in impaired growth with xanthine. hyp5 strains are unable to convert both hypoxanthine and xanthine to uric acid. The mutations are recessive and none of the loci are linked to each other. The possible catalytic steps involved are discussed.
嘌呤类物质，如次黄嘌呤、黄嘌呤、尿酸、尿囊素和尿囊酸，可作为酵母裂殖酵母的唯一氮源。已经分离和遗传分析了许多不能利用嘌呤的突变体。uro1 基因的突变体缺乏尿酸酶，all1 缺乏尿囊素酶，ala1 缺乏尿囊酸酶，而 ure1、ure2、ure3 和 ure4 基因缺乏脲酶活性。四个 hyp 基因的突变体不能将次黄嘌呤转化为尿酸，而 xan1 的突变导致黄嘌呤生长受损。hyp5 菌株不能将次黄嘌呤和黄嘌呤都转化为尿酸。这些突变是隐性的，而且这些基因座彼此之间没有连锁。讨论了可能涉及的催化步骤。
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