Li Xiaohua, Zhang Zhujun, Tao Liang, Li Yongbo, Li Yunyun
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, China.
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2014;120:67-71. doi: 10.1016/j.saa.2013.10.012. Epub 2013 Oct 12.
A new chemiluminescent (CL) probe array assay approach was first developed. The new CL probe array was based on Co3O4-SiO2 mesoporous nanocomposite material, which not only has an excellent catalytic effect on the luminol-H2O2 CL reaction in alkaline medium but also can be used for the immobilization of enzymes. As a model, the novel bifunctional CL probe array has been applied to the high-throughput determination of glucose in human. The linear range of the glucose concentration was 3-90 μM and the detection limit was 0.36 μM. It breaks traditional development view in solid phase supports and provides new insights into the application of mesoporous material.
首次开发了一种新的化学发光（CL）探针阵列检测方法。新型CL探针阵列基于Co3O4-SiO2介孔纳米复合材料，该材料不仅在碱性介质中对鲁米诺-H2O2化学发光反应具有优异的催化作用，而且可用于酶的固定化。作为一个模型，新型双功能CL探针阵列已应用于人体葡萄糖的高通量检测。葡萄糖浓度的线性范围为3-90μM，检测限为0.36μM。它打破了传统的固相载体开发观念，为介孔材料的应用提供了新的见解。
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