Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada.
Plant Cell Rep. 1996 Mar;15(7):516-21. doi: 10.1007/BF00232985.
A simple and efficient procedure suitable for extraction of high-quality RNA from cultured conifer tissues, somatic embryos, zygotic embryos, needles, stem and root tissues was developed. It produced from 100 μg up to 700 μg total RNA per gram tissue dependent on the types of tissues used. RNA quality was estimated by spectrophotometry, agarose gel electrophoresis, in vitro translation of mRNA, cDNA synthesis and Northern blot analysis. The method also worked well with Arabidopsis thaliana and tobacco tissues.
开发了一种简单高效的程序，适用于从培养的针叶组织、体细胞胚、合子胚、针叶、茎和根组织中提取高质量 RNA。根据使用的组织类型，每克组织可产生 100μg 至 700μg 总 RNA。通过分光光度法、琼脂糖凝胶电泳、mRNA 体外翻译、cDNA 合成和 Northern 印迹分析来估计 RNA 的质量。该方法也适用于拟南芥和烟草组织。
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