从商业品种的龙胆属植物的叶片、茎和根外植体再生不定芽。

Adventitious shoot regeneration from leaf, stem and root explants of commercial cultivars of Gentiana.

机构信息

Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, 024, Iwate, Japan.

出版信息

Plant Cell Rep. 1996 Apr;15(8):578-81. doi: 10.1007/BF00232456.

Abstract

Several culture conditions were examined for promoting efficient plant regeneration from explants of Gentiana. Adventitious shoot regeneration from leaf explants of cv. WSP-3 was very superior on MS medium, compared to B5 medium, supplemented with four cytokinins (TDZ, 4PU-30, BA and zeatin). An auxin / cytokinin combination was required for regeneration. TDZ was the most effective cytokinin, while NAA was more effective than IAA or 2,4-D. Optimum conditions for regeneration from explants (leaf, stem and root) of cv. WSP-3, evaluated in terms of regeneration frequency and number of regenerated shoots per explant, were TDZ and NAA in combination, 5-10 mg/l and 0.1 mg/l for leaf and stem explants, and 10 mg/l and 1 mg/l for root explants, respectively. Application of these conditions to eight other commercial cultivars resulted in 30-100% regeneration from leaf explants. The number of chromosomes in each of ten regenerated plants of each cultivar was diploid, 2n=26. Shoots regenerated in vitro were rooted in phytohormone-free medium and transferred to soil.

摘要

考察了几种培养条件以促进从秦艽外植体中高效地进行植物再生。与 B5 培养基相比，WSP-3 品种的叶片外植体在 MS 培养基上，添加四种细胞分裂素（TDZ、4PU-30、BA 和玉米素）时，不定芽再生非常优异。再生需要生长素/细胞分裂素组合。TDZ 是最有效的细胞分裂素，而 NAA 比 IAA 或 2,4-D 更有效。从 WSP-3 品种的外植体（叶片、茎和根）评估的再生最佳条件（以再生频率和每个外植体再生的芽数衡量）是 TDZ 和 NAA 的组合，叶片和茎外植体的浓度为 5-10mg/l 和 0.1mg/l，根外植体的浓度分别为 10mg/l 和 1mg/l。将这些条件应用于另外 8 个商业品种，导致叶片外植体的再生率为 30-100%。每个品种的 10 株再生植株的染色体数目均为二倍体，2n=26。体外再生的芽在无植物激素的培养基中生根，然后移栽到土壤中。