N(5)-(羧甲基)-L-鸟氨酸和 N (6)-(羧甲基)-L-赖氨酸的酶法合成与表征。
Enzymatic synthesis and characterization of N(5)-(carboxymethyl)-L-ornithine and N (6)-(carboxymethyl)-L-lysine.
机构信息
Developmental and Metabolic Neurology Branch, National Institute of Neurological Disorders and Stroke, 9000 Rockville Pike, 20892, Bethesda, MD, USA.
出版信息
Amino Acids. 1994 Jun;6(2):189-98. doi: 10.1007/BF00805846.
This report describes the enzyme-catalyzed synthesis, characterization, and chromatographic separation of N(6)-(carboxymethyl)-L-lysine and N(5)-(carboxymethyl)-L-ornithine. The two N (ω) -(carboxyalkyl)amino acids are formed via a reductive condensation between glyoxylate and theε- orδ-amino groups of lysine and ornithine, respectively. Both reactions are catalyzed by the NADPH-dependent enzyme, N(5)-(carboxyethyl)ornithine synthase [EC 1.5.1.24], found in some strains of the lactic acid bacteriumLactococcus lactis subsp.lactis.
本报告描述了 N(6)-(羧甲基)-L-赖氨酸和 N(5)-(羧甲基)-L-鸟氨酸的酶催化合成、表征和色谱分离。这两种 N(ω)-(羧烷基)氨基酸分别通过乙醛酸与赖氨酸和鸟氨酸的ε-或δ-氨基之间的还原缩合形成。这两个反应都是由 NADPH 依赖性酶 N(5)-(羧乙基)鸟氨酸合酶[EC 1.5.1.24]催化的，该酶存在于某些乳酸乳球菌亚种乳球菌的菌株中。
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