水禽中抗甲型 H5 亚型流感病毒抗体的商业酶联免疫吸附试验评估。
Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl.
机构信息
Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, The University of Georgia, Athens, GA, USA.
出版信息
Influenza Other Respir Viruses. 2013 Nov;7(6):1237-40. doi: 10.1111/irv.12070. Epub 2012 Dec 22.
The ID Screen Influenza H5 Antibody Competition enzyme-linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domestica), experimentally infected with eight low pathogenic (LP) and nine highly pathogenic (HP) H5N1 IA viral strains. Three incubation periods (1, 4 and 18 hours) and two dilutions (1:2 and 1:5) were tested. All serum samples from LP H5-infected birds tested positive; however, improved detection rates were observed for viruses belonging to the HP H5N1 clade 2.2.1 as compared with those belonging to clade 2.1.3.
ID Screen Influenza H5 抗体竞争酶联免疫吸附试验用于检测水禽中甲型流感病毒（IA）H5 亚型的抗体。使用经 8 株低致病性（LP）和 9 株高致病性（HP）H5N1IA 病毒株人工感染的 Mallards（Anas platyrhynchos）和 Pekin Ducks（Anas platyrhynchos domestica）血清进行了检测。测试了三个孵育期（1、4 和 18 小时）和两个稀释度（1:2 和 1:5）。所有 LP H5 感染鸟类的血清样本均呈阳性反应；然而，与属于 clade 2.1.3 的病毒相比，属于 clade 2.2.1 的 HP H5N1 病毒的检测率有所提高。
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