四倍体孤挺花（A. pelegrina × A. psittacina）成熟合子胚的愈伤组织诱导和植株再生。

Callus induction and plant regeneration from mature zygotic embryos of a tetraploid Alstroemeria (A. pelegrina × A. psittacina).

机构信息

Department of Horticultural Sciences, University of Guelph, N1G 2W1, Guelph, Ontario, Canada.

出版信息

Plant Cell Rep. 1994 Dec;14(2-3):184-7. doi: 10.1007/BF00233787.

PMID:24192891

Abstract

A simple procedure was developed to induce callus growth and whole plant regeneration for a tetraploid cultivar of Alstroemeria. The callus, induced from mature zygotic embryos cultured on a medium supplemented with 20 μM kinetin with 10 or 20 μM NAA, could be maintained for one year without any loss of regeneration potential. Maximum frequency of regeneration (40%) was obtained with calli maintained on the medium containing 20 μM kinetin and 20 μM NAA. Whole plant regeneration occurred via somatic embryogenesis in the absence of growth regulators and the plantlets grew to maturity and flowered in the greenhouse conditions.

摘要

建立了一个简单的程序，以诱导四倍体孤挺花的愈伤组织生长和植株再生。从成熟的合子胚在添加 20μM 激动素和 10 或 20μM NAA 的培养基上培养诱导的愈伤组织，可以在不丧失任何再生潜力的情况下维持一年。在含有 20μM 激动素和 20μM NAA 的培养基上维持的愈伤组织可获得最高的再生频率（40%）。在没有生长调节剂的情况下，通过体细胞胚胎发生实现了植株再生，并且在温室条件下，植株生长成熟并开花。