鹰嘴豆愈伤组织器官发生途径的植株再生。

Whole plant regeneration of Cicer arietinum from callus cultures via organogenesis.

机构信息

Department of Biosciences, University of Jammu, 180004, Jammu, India.

出版信息

Plant Cell Rep. 1994 Jun;13(9):510-3. doi: 10.1007/BF00232946.

PMID:24194130

Abstract

A protocol for whole plant regeneration of Cicer arietinum L. cv. C-235 via organogenesis from callus has been developed. Callus initiation was best when immature leaflets were cultured on MS medium containing 5 or 25 μM 2,4-D or NAA in combination with 10 μM BA, or 25 μM 2,4-D alone. The callus grew most vigorously on MS medum supplemented with 10 μM NAA and 5 μMBA. Best shoot differentiation was obtained from calli derived from the basal portion of shoot tips on MS medium supplemented with 10 μM BA and 0.1 μM IBA. The shoot forming ability of calli was enhanced by adding 5 mM potassium phosphate to the medium. Shoots were rooted on a MS medium containing l μM IBA. The regenerated plants were grown to maturity and produced viable seed.

摘要

已制定通过愈伤组织器官发生从鹰嘴豆 cv.C-235 中再生植株的整体方案。当在含有 5 或 25μM 2,4-D 或 NAA 与 10μM BA，或单独 25μM 2,4-D 的 MS 培养基上培养未成熟的小叶时，愈伤组织的起始最好。在补充有 10μM NAA 和 5μMBA 的 MS 培养基上，愈伤组织生长最旺盛。从在补充有 10μM BA 和 0.1μM IBA 的 MS 培养基上的茎尖基部衍生的愈伤组织中获得最佳的芽分化。通过向培养基中添加 5mM 磷酸钾可增强愈伤组织的成芽能力。芽在含有 1μM IBA 的 MS 培养基上生根。再生植株生长成熟并产生有活力的种子。