蓝藻分离株的细胞结合和细胞外磷酸酶活性。

Cell-bound and extracellular phosphatase activities of cyanobacterial isolates.

机构信息

Department of Biological Sciences, University of Durham, DH1 3LE, Durham, UK.

出版信息

Microb Ecol. 1991 Dec;21(1):85-98. doi: 10.1007/BF02539146.

PMID:24194203

Abstract

Fifty cyanobacterial strains (10 genera) were tested in batch culture for their ability to use organic phosphorus compounds (1 mg liter(-1) P) as their sole P source. Two monoesters, Na2-β-glycerophosphate and π-nitrophenyl phosphate (πNPP), supported growth of all strains, and the diester bis-π-nitrophenyl phosphate (bis-π-NPP) and herring sperm DNA supported almost all strains. ATP was either a very favorable or poor P source and failed to support growth of nine strains, seven of which were Rivulariaceae with trichomes ending in a hair or long tapered region. Phytic acid was in general the least favorable P source.P-limited cultures grown initially with inorganic phosphate to conditions of P limitation were also tested for cell-bound and extracellular phosphomonoesterase (PMEase) and phosphodiesterase (PDEase) activities at two pH values (7.6, 10.3) using πNPP and bis-πNPP as substrates. Cell-bound PMEase was inducible in all strains and cell-bound PDEase in most strains. Most showed extracellular PMEase, but not extracellular PDEase. The highest values (μM πNPP or bis-πNPP hydrolyzed mg dry weight(-1) hour(-1)) all occurred in strains ofGloeotrichia as follows: cell-bound PMEase at pH 7.6, 2.7 μM in strain D602; cell-bound PMEase at pH 10.3, 5.2 μM in D602; extracellular PMEase at pH 7.6, 0.73 μM in D281; extracellular PMEase at pH 10.3, 6.6 μM in D281; cell-bound PDEase at 7.6, 0.40 μM in D613; cell-bound PDEase at pH 10.3, 1.0 μM in D613.The results were compared to see if they indicated possible relationships between phosphatase activity and taxonomic or ecological grouping. The following differences were significant (P<0.05). Rivulariaceae produced higher yields than filamentous non-Rivulariaceae with β-glycerophosphate, πNPP, and DNA. Rivulariaceae with the ability to form hairs in culture showed poorer growth in ATP than non-hair-forming Rivulariaceae, but were more effective at utilizing phytic acid. Strains from calcareous environments had higher PMEase activity at pH 10.3 than strains from noncalcareous environments (P<0.01).

摘要

对 50 株蓝藻（10 属）进行了分批培养实验，以测试它们利用有机磷化合物（1mgL(-1)P）作为唯一磷源的能力。两种单酯，Na2-β-甘油磷酸和π-硝基苯磷酸盐（πNPP），支持所有菌株的生长，双酯双-π-硝基苯磷酸盐（双-π-NPP）和鲱鱼精子 DNA 几乎支持所有菌株的生长。ATP 是一种非常有利或较差的磷源，不能支持 9 株菌的生长，其中 7 株属于 Rivulariaceae，其毛状体末端为毛发或长锥形区域。植酸通常是最不利的磷源。用无机磷最初培养到磷限制条件下的磷限制培养物，也用πNPP 和双-π-NPP 作为底物，在两个 pH 值（7.6、10.3）下测试细胞结合和细胞外磷酸单酯酶（PMEase）和磷酸二酯酶（PDEase）活性。所有菌株都可诱导细胞结合 PMEase，大多数菌株可诱导细胞结合 PDEase。大多数菌株表现出细胞外 PMEase，但没有细胞外 PDEase。最高值（μMπNPP 或双-π-NPP 水解 mg 干重(-1)小时(-1)）均出现在如下以下属的 Glcothrichia 菌株中：细胞结合 PMEase 在 pH 7.6 时为 2.7μM，在菌株 D602 中；细胞结合 PMEase 在 pH 10.3 时为 5.2μM，在 D602 中；细胞外 PMEase 在 pH 7.6 时为 0.73μM，在 D281 中；细胞外 PMEase 在 pH 10.3 时为 6.6μM，在 D281 中；细胞结合 PDEase 在 pH 7.6 时为 0.40μM，在 D613 中；细胞结合 PDEase 在 pH 10.3 时为 1.0μM，在 D613 中。将结果进行比较，以观察它们是否表明磷酸酶活性与分类学或生态学分组之间存在可能的关系。以下差异具有统计学意义（P<0.05）。β-甘油磷酸、πNPP 和 DNA 培养物中 Rivulariaceae 的产率高于丝状非 Rivulariaceae。在培养中能够形成毛发的 Rivulariaceae 比不能形成毛发的 Rivulariaceae 在 ATP 中的生长较差，但更有效地利用植酸。来自石灰质环境的菌株在 pH 10.3 时的 PMEase 活性高于非石灰质环境的菌株（P<0.01）。