Departments of Ophthalmology and Pharmacology, College of Physicians and Surgeons, Columbia University, New York, New York.
Inflammation. 1976 Mar;1(2):117-25. doi: 10.1007/BF00917523.
Intracameral injections of bradykinin into the eyes of rabbits anesthetized with urethane were found to produce a dose-dependent constriction of the pupil and increase in the amount of protein present in the aqueous humor. Both these effects were relatively fast in onset, pupillary constriction being observed 1-2 min after the injection. The intact bradykinin molecule was required to produce these effects since prior incubation of known amounts of bradykinin with chymotrypsin and subsequent intracameral injection were without effect. No kininase activity was observed in samples of normal aqueous humor, however, kininase activity was present in aqueous humor removed from eyes inflamed by either paracentesis or nitrogen mustard. The actions of bradykinin on both the pupil and the protein content of aqueous humor were markedly reduced or abolished by pretreatment with inhibitors of prostaglandin biosynthesis, such as indomethacin or pirprofen, given either topically or by intraperitoneal injection. In these animals the simultaneous injection of prostaglandin Ez together with the bradykinin restored the ocular responses to normal. These results suggest that prostaglandins contribute to the ocular actions of bradykinin.
在氨基甲酸乙酯麻醉的兔子眼睛内房水中注射缓激肽,发现其可引起瞳孔的剂量依赖性收缩,并增加房水中蛋白质的含量。这两种作用的起效都很快,注射后 1-2 分钟即可观察到瞳孔收缩。完整的缓激肽分子是产生这些作用所必需的,因为预先用糜蛋白酶孵育已知量的缓激肽,然后再进行眼内注射,没有效果。然而,在通过前房穿刺或氮芥引起的炎症眼的房水中存在激肽酶活性。在经皮或腹腔内注射给予前列腺素生物合成抑制剂,如吲哚美辛或吡罗昔康预处理后,缓激肽对瞳孔和房水蛋白含量的作用明显降低或消除。在这些动物中,同时注射前列腺素 E2 可使缓激肽的眼部反应恢复正常。这些结果表明,前列腺素有助于缓激肽的眼部作用。