Dept. of Biochemistry, Searle Research Laboratories, High Wycombe, Bucks., England.
Mol Biol Rep. 1973 Oct;1(3):129-34. doi: 10.1007/BF00357152.
Messenger RNAs for 4 egg white proteins (ovalbumin, conalbumin, ovomucoid and lysozyme) were assayed in a cell-free, protein-synthesizing system derived from rabbit reticulocytes. Each of these messengers was purified about 33-fold from hen oviduct polysomal RNA using oligo(dT) cellulose. The apparent minimal translational efficiencies varied from 22 translations for each ovalbumin mRNA to less than 1 for lysozyme mRNA. These messengers are not polycistronic with each other since they have distinct sedimentation values of: lysozyme, 8.5S; ovomucoid, 11S; ovalbumin, 15S; and conalbumin 18S. Several aspects of this system indicate that it will be valuable for dissecting the fine control of mRNA metabolism.
卵清白蛋白（卵白蛋白、伴白蛋白、卵类粘蛋白和溶菌酶）的信使 RNA 在兔网织红细胞无细胞蛋白质合成系统中进行了检测。使用寡聚（dT）纤维素从母鸡输卵管多核糖体 RNA 中每种信使 RNA 都纯化了约 33 倍。每个卵白蛋白 mRNA 的明显最小翻译效率从 22 次翻译变化到溶菌酶 mRNA 的不到 1 次。这些信使 RNA 彼此之间不是多顺反子的，因为它们具有不同的沉降值：溶菌酶，8.5S；卵类粘蛋白，11S；卵白蛋白，15S；伴白蛋白，18S。该系统的几个方面表明，它将有助于剖析 mRNA 代谢的精细调控。
Zotero 插件