Zeng Xinping, Zhang Min, Li Weihao, Li Chang, Tang Wenwei
School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Biomed Mater Eng. 2014;24(1):931-7. doi: 10.3233/BME-130887.
A strain of Pseudomonas aeruginosa (Pseudomonas sp. R1), which can efficiently decolorize reactive red X-3B, was isolated from activated sludge in a dye plant, and the decolorizing mechanism was explored in this paper. The result shows that Pseudomonas sp. R1 has very good capability for decolorization of reactive red X-3B and the decolorization rate is increased by 9.1% after optimization of the experimental parameters, which means that 89.6% of the reactive red can be removed. Investigation on decolorization mechanism showed that the decolorizing capability of Pseudomonassp. R1 was significantly affected after plasmids in Pseudomonassp. R1 were eliminated by acridine orange (AO). Meanwhile, E. coli DH5a could gain decolorizing capability after transformed with the plasmids. Plasmid elimination and transformation tests proved that the decolorizing gene in Pseudomonas sp. R1 exists in the plasmid.
从一家染料厂的活性污泥中分离出了一株能高效使活性艳红X-3B脱色的铜绿假单胞菌（假单胞菌属R1），并对其脱色机制进行了研究。结果表明，假单胞菌属R1对活性艳红X-3B具有很好的脱色能力，优化实验参数后脱色率提高了9.1%，即89.6%的活性艳红可被去除。对脱色机制的研究表明，用吖啶橙（AO）消除假单胞菌属R1中的质粒后，其脱色能力受到显著影响。同时，大肠杆菌DH5α用这些质粒转化后可获得脱色能力。质粒消除和转化试验证明，假单胞菌属R1中的脱色基因存在于质粒中。
