胡椒科植物长胡椒愈伤组织器官发生再生植株。

Plant regeneration from callus cultures of Piper longum L. by organogenesis.

机构信息

National Plant Tissue Culture Repository, NBPGR, Pusa Campus, 110012, New Delhi, India.

出版信息

Plant Cell Rep. 1992 Sep;11(10):525-8. doi: 10.1007/BF00236270.

Abstract

Plant regeneration from callus cultures of Piper longum was achieved through organogenesis. In vitro grown shoots were used as explants for callus induction. Competent callus was initiated around the nodal ring of tissue using Murashige and Skoog medium supplemented with 1.0 mg.l(-1)α- naphthaleneacetic acid and 0.2 mg.l(-1) N(6)-benzyladenine. Optimum growth regulator concentrations for shoot induction and shoot elongation were found to be 0.5 mg.l(-1) indole-3-acetic acid with 1.5 mg.l(-1) benzyladenine, and 0.1 mg.l(-1) indole-3-acetic acid with 0.2 mg.l(-1) benzyladenine, respectively. Elongated shoots were rooted on half-strength Murashige and Skoog medium having 0.1 mg.l(-1) indole3-acetic acid. The rooted plants were successfully established in soil.

摘要

胡椒（Piper longum）愈伤组织通过器官发生实现植株再生。体外培养的芽用作诱导愈伤组织的外植体。使用添加 1.0 mg.l(-1)α-萘乙酸和 0.2 mg.l(-1)N(6)-苄基腺嘌呤的 Murashige 和 Skoog 培养基，在组织的节环周围起始有能力的愈伤组织。发现用于芽诱导和芽伸长的最佳生长调节剂浓度分别为 0.5 mg.l(-1)吲哚-3-乙酸和 1.5 mg.l(-1)苄基腺嘌呤，以及 0.1 mg.l(-1)吲哚-3-乙酸和 0.2 mg.l(-1)苄基腺嘌呤。伸长的芽在含有 0.1 mg.l(-1)吲哚-3-乙酸的半强度 Murashige 和 Skoog 培养基上生根。生根的植物成功地在土壤中建立。