[亚慢性砷暴露小鼠体内砷形态分布及其DNA损伤]

[Distribution of arsenic species and its DNA damage in subchronic arsenite-exposed mice].

作者信息

Li Hanjun, Lin Jing, Li Yufeng, Yan Jinting, Li Bai, Zhang Wei, Dong Zeqin, Chen Chunying

机构信息

Multi-Discipline Initiative Center, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.

出版信息

Wei Sheng Yan Jiu. 2013 Sep;42(5):764-9, 776.

PMID:24218882

Abstract

OBJECTIVE

To study the arsenic distribution, speciation, its effects on the balance of other elements and the DNA damage by subchronic arsenite exposure in mice.

METHODS

The 8-week-old C57BL/6N mice were matched by weight and divided into control group and supplementation group, which were given 0 or 10 microg/ml of sodium arsenite in the drinking water, and continuous exposed for 6 months.

RESULTS

Arsenic was found in various tissues and organs. The highest ones were in the kidney, lung and liver, reached (563.9 +/- 222.5), (458.6 +/- 191.0) and (279.8 +/- 81.2) ng/g, respectively while the lowest in the blood and brain, reached (82.2 +/- 26.7) ng/ml and (101.8 +/- 30.1) ng/g, respectively. Arsenic exists mainly in the form of dimethylarsinous acid (DMA). Compared to the control group, there was a significant difference (P < 0.05) between arsenic and chromium, copper, zinc, selenium, lead in some organs of arsenic exposed group, but not cadmium. Furthermore, the 8-hydroxydeoxyguanosine (8-OHdG) level of the exposed group was (149.1 +/- 1.0) ng/ml, which was significantly higher than the control group of (76.4 +/- 27.9) ng/ml.

CONCLUSION

Arsenic accumulated in various tissues and organs mainly in the form of DMA, which affected the balance of chromium, copper, zinc, selenium and lead in the body, and led to DNA damage after subchronic exposure.

摘要

目的

研究亚慢性砷暴露对小鼠体内砷分布、形态、对其他元素平衡的影响以及DNA损伤情况。

方法

将8周龄C57BL/6N小鼠按体重匹配，分为对照组和补砷组，分别给予含0或10μg/ml亚砷酸钠的饮用水，连续暴露6个月。

结果

砷在各组织器官中均有发现。含量最高的是肾脏、肺和肝脏，分别达到(563.9±222.5)、(458.6±191.0)和(279.8±81.2)ng/g，而血液和脑中最低，分别达到(82.2±26.7)ng/ml和(101.8±30.1)ng/g。砷主要以二甲基砷酸（DMA）的形式存在。与对照组相比，砷暴露组部分器官中砷与铬、铜、锌、硒、铅之间存在显著差异（P<0.05），但与镉无差异。此外，暴露组的8-羟基脱氧鸟苷（8-OHdG）水平为(149.1±1.0)ng/ml，显著高于对照组的(76.4±27.9)ng/ml。