Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.
Proteomics. 2014 Jan;14(1):116-20. doi: 10.1002/pmic.201300237.
In this study, we report the first dataset of phosphoproteins of the seeds of a model plant, Lotus japonicus. This dataset might be useful in studying the regulatory mechanisms of seed germination in legume plants. By proteomic analysis of seeds following water absorption, we identified a total of 721 phosphopeptides derived from 343 phosphoproteins in cotyledons, and 931 phosphopeptides from 473 phosphoproteins in hypocotyls. Kinase-specific prediction analyses revealed that different kinases were activated in cotyledons and hypocotyls. In particular, many peptides containing ATM-kinase target motifs, X-X-pS/pT-Q-X-X, were detected in cotyledons. Moreover, by real-time RT-PCR analysis, we found that expression of a homolog of ATM kinase is upregulated specifically in cotyledons, suggesting that this ATM-kinase homolog plays a significant role in cell proliferation in the cotyledons of L. japonicus seeds. The data have been deposited to the ProteomeXchange with identifier PXD000053 (http://proteomecentral.proteomexchange.org/dataset/PXD000053).
在这项研究中，我们报告了第一个模式植物种子的磷酸化蛋白质数据集。该数据集可能有助于研究豆科植物种子萌发的调控机制。通过吸水后对种子进行的蛋白质组学分析，我们在子叶中鉴定出了总共 343 个磷酸化蛋白的 721 个磷酸肽，在胚轴中鉴定出了 473 个磷酸化蛋白的 931 个磷酸肽。激酶特异性预测分析表明，不同的激酶在子叶和胚轴中被激活。特别是，在子叶中检测到许多含有 ATM-激酶靶标基序 X-X-pS/pT-Q-X-X 的肽。此外，通过实时 RT-PCR 分析，我们发现 ATM 激酶同源物的表达在子叶中特异性地上调，表明该 ATM-激酶同源物在 L. japonicus 种子的子叶细胞增殖中发挥重要作用。该数据集已被存入 ProteomeXchange，标识符为 PXD000053（http://proteomecentral.proteomexchange.org/dataset/PXD000053）。
