具有可育和不育细胞质的高粱线粒体 DNA 的限制性内切酶分析。

Restriction endonuclease analysis of mitochondrial DNA from sorghum with fertile and male-sterile cytoplasms.

机构信息

Departments of Genetics, Biochemistry and Agronomy, Kansas State University, 66506, Manhattan, KS, USA.

出版信息

Theor Appl Genet. 1989 Mar;77(3):379-82. doi: 10.1007/BF00305832.

Abstract

Mitochondrial DNA from four paired (fertile and male-sterile) lines and six isocytoplasmic strains of sorghum (Sorghum bicolor (L.) Moench) were fragmented by endonucleases and their electrophoretic patterns were examined. Cytoplasmic male sterile lines differed from their male-fertile counterparts consistently. Among the isocytoplasmic strains, KS 36A (S. verticilli-florum cytoplasm), KS 38A (S. conspicum cytoplasm), and KS 39A (S. niloticum cytoplasm) showed minor differences from the other strains. Results suggest that restriction endonuclease patterns are useful in detecting differences in mitochondrial genomes.

摘要

从四个配对（可育和雄性不育）系和六个同细胞质的高粱（高粱 bicolor (L.) Moench）品系中提取线粒体 DNA，用内切酶进行片段化，并检测其电泳图谱。细胞质雄性不育系与可育系始终存在差异。在同细胞质的品系中，KS 36A（高粱 verticilli-florum 细胞质）、KS 38A（高粱 conspicum 细胞质）和 KS 39A（高粱 niloticum 细胞质）与其他品系相比存在较小差异。结果表明，限制内切酶图谱可用于检测线粒体基因组的差异。