反相高效液相色谱法测定土壤中甲拌磷。
Determination and method validation of metamitron in soil by RP-HPLC.
机构信息
Divison of Agricultural Chemicals, Department of Chemistry, College of Basic Sciences and Humanities, G.B Pant University of Agriculture and Technology, Pantnagar, 263145, Uttarakhand, India,
出版信息
Bull Environ Contam Toxicol. 2014 Feb;92(2):165-8. doi: 10.1007/s00128-013-1151-z. Epub 2013 Nov 16.
A simple, rapid and economical method was developed and validated for the determination of metamitron using UV detector with RP-HPLC. Study of metamitron in soil was carried out. The compound was extracted from soil by methanol and clean-up was done on C-18 SPE column. Recovery ranged from 90.75 % to 94.05 % within 0.1-2.0 μg g(-1) and RSD 1.80 %. Retention time was 3.8 min and limit of detection and limit of quantification were 0.001 and 0.008 μg g(-1). The results indicated that the reported method could meet the requirement for the analysis of metamitron in trace amounts.
本研究建立了一种使用反相高效液相色谱法（RP-HPLC）结合紫外检测器测定甲噻隆的方法。该方法简单、快速、经济。对甲噻隆在土壤中的残留情况进行了研究。采用甲醇提取土壤中的甲噻隆，然后用 C-18 SPE 柱进行净化。在 0.1-2.0 μg g(-1)浓度范围内，回收率为 90.75%-94.05%，相对标准偏差为 1.80%。保留时间为 3.8 min，检测限和定量限分别为 0.001 和 0.008 μg g(-1)。结果表明，该方法能够满足痕量甲噻隆分析的要求。
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