Department of Physiology, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Copenhagen Valby, Denmark.
Planta. 1984 Dec;162(6):487-94. doi: 10.1007/BF00399913.
Experimental evidence for a membranebound microsomal ester synthetase from Bonus barley primary leaves is reported. The results are consistent with at least two mechanisms for the synthesis of barley wax esters: an acyl-CoA-fattyalcohol-transacylase-type reaction and an apparent direct esterification of alcohols with fatty acids. Biosynthesis of wax esters was not specific with regard to the chain length of the tested alcohols. The microsomal preparation readily catalyzed the esterification of C16-, C18-, C22- or C24-labelled alcohols with fatty acids of endogenous origin. Exogenous long-chain alcohols were exclusively incorporated into the alkyl moieties of the esters. Addition of ATP, CoA and-or free fatty acids was not effective in stimulating or depressing the esterifying activity of the microsomal fraction. Partial solubilization of the ester synthetase was obtained using phosphate-buffered saline.
实验证据表明，大麦初生叶片中有膜结合的微粒体酯合成酶。这些结果与大麦蜡酯合成的至少两种机制相符：酰基辅酶 A-脂肪酸-醇转酰基酶型反应和醇与脂肪酸的明显直接酯化作用。就所测试的醇的链长而言，蜡酯的生物合成没有特异性。微粒体制剂可轻易地催化 C16、C18、C22 或 C24 标记醇与内源脂肪酸的酯化作用。外源性长链醇仅被掺入到酯的烷基部分。添加 ATP、CoA 和/或游离脂肪酸不能有效地刺激或抑制微粒体部分的酯化活性。用磷酸盐缓冲盐水可获得酯合成酶的部分溶解。
