Department of Botany, Faculty of Science, University of Zagreb, P.O. Box 933, 41001, Zagreb, Yugoslavia.
Plant Cell Rep. 1984 Aug;3(4):125-9. doi: 10.1007/BF00270204.
Callus tissue was induced on barley mesocotyl explants of germinated seven-day-old seedlings on MS medium supplemented with 2,4-D or 2,4,5-T in high concentrations. Two morphologically different tissue cultures were maintained in vitro for a long time: a callus tissue without organogenesis and a culture with high rhizogenic capacity. Shoots and plantlets were generated when the auxin-media induced callus was transferred to medium supplemented with 3 μM TIBA. In 62% of cultures, during the first five subcultures, four to twentyeight plants per single mesocotyl were obtained. Some cultures produced shoots even in the 9th subculture, being in culture for nearly 14 months. The largest number of plants obtained per one mesocotyl was forty. Plantlets rooted well on MS with 5.7 μM IAA and survived transplantation to soil in high percentages.
在 MS 培养基上，用高浓度的 2,4-D 或 2,4,5-T 诱导发芽 7 天的大麦中胚轴外植体的愈伤组织。在体外长时间维持两种形态上不同的组织培养物：一种无器官发生的愈伤组织和一种具有高生根能力的培养物。当将含有生长素的培养基诱导的愈伤组织转移到添加 3 μM TIBA 的培养基上时，会产生芽和小植株。在 62%的培养物中，在前五次继代培养中，从单个中胚轴获得了四到二十八株植物。一些培养物甚至在第九次继代培养中也产生了芽，培养时间将近 14 个月。从一个中胚轴获得的最大植物数量为四十个。小植株在含有 5.7 μM IAA 的 MS 上生根良好，移植到土壤中的存活率很高。
