萌发玉米胚中的氨基酸池和蛋白质合成。

Amino acid pools and protein synthesis in germinating maize embryos.

机构信息

Plant Biochemistry Department, Facultad de Quimica, Universidad Nacional Autonoma de Mexico, 04510, Mexico, DF.

出版信息

Plant Cell Rep. 1984 Oct;3(5):193-5. doi: 10.1007/BF00270198.

Abstract

Amino acid pools were analyzed in maize (Zea mays L.) axes germinated for 0, 6 and 24 h. Proteins synthesized at early (0-6 h) and late (18-24 h) stages were characterized by gel electrophoresis and fluorography after either (14)C-leucine or (14)C-lysine pulses. An increase of amino acid incorporation after 18-24 h was observed, as well as changes in the protein patterns of the corresponding fluorographs. Analysis of the endogenous amino acid pools showed major changes in contents of proline, alanine, isoleucine, valine, leucine and lysine. A selective increase of lysine incorporation into proteins during the late stage was detected.

摘要

对萌发 0、6 和 24 小时的玉米（Zea mays L.）轴进行了氨基酸池分析。用（14）C-亮氨酸或（14）C-赖氨酸脉冲处理后，通过凝胶电泳和放射自显影对早期（0-6 小时）和晚期（18-24 小时）合成的蛋白质进行了表征。在 18-24 小时后观察到氨基酸掺入的增加，以及相应放射自显影图谱的蛋白质模式的变化。对内源性氨基酸池的分析表明脯氨酸、丙氨酸、异亮氨酸、缬氨酸、亮氨酸和赖氨酸的含量发生了重大变化。在晚期检测到赖氨酸掺入蛋白质的选择性增加。