肉碱长链脂酰转移酶和豌豆线粒体制剂中棕榈酸、棕榈酰辅酶 A 和棕榈酰肉碱的氧化。

Carnitine long-chain acyltransferase and oxidation of palmitate, palmitoyl coenzyme A and palmitoylcarnitine by pea mitochondria preparations.

机构信息

Department of Plant Biology, Newcastle University, NE1 7RU, Newcastle upon Tyne, UK.

出版信息

Planta. 1984 May;161(3):255-60. doi: 10.1007/BF00982922.

Abstract

Palmitoylcarnitine was oxidised by pea mitochondria.L-carnitine was an essential addition for the oxidation of palmitate or palmitoylCoA. When palmitate was sole substrate, ATP and Mg(2+) were also essential additives for maximum oxidation. Additions of CoA inhibited the oxidation of palmitate. It was shown that CoA was acting as a competitive inhibitor of the carnitine-stimulated O2 uptake. It is suggested that palmitoylacarnitine and carnitine passed through the mitochondrial barrier with ease but palmitoylCoA and CoA did not. The presence of carnitine long-chain acyl (palmitoyl)transferase (EC 2.3.1.21) in pea-cotyledon mitochondria was shown. This enzyme may play a role in the transport of long-chain acyl groups through membrane barriers.

摘要

棕榈酰肉碱被豌豆线粒体氧化。L-肉碱是棕榈酸或棕榈酰 CoA 氧化所必需的添加物。当棕榈酸是唯一的底物时，ATP 和 Mg(2+)也是最大氧化所必需的添加物。CoA 的添加抑制了棕榈酸的氧化。结果表明，CoA 作为肉碱刺激的 O2 摄取的竞争性抑制剂发挥作用。这表明棕榈酰肉碱和肉碱很容易穿过线粒体屏障，但棕榈酰 CoA 和 CoA 则不然。证明了豌豆子叶线粒体中存在肉碱长链酰基（棕榈酰基）转移酶（EC 2.3.1.21）。这种酶可能在长链酰基通过膜屏障的运输中发挥作用。