宏观营养素对西洋菜悬浮细胞生长和迷迭香酸形成的影响。

Effects of macronutrients on growth and rosmarinic acid formation in cell suspension cultures of Anchusa officinalis.

机构信息

Guelph-Waterloo Centre for Graduate Work in Chemistry, University of Guelph, N1G 2W1, Guelph, Ontario, Canada.

出版信息

Plant Cell Rep. 1985 Apr;4(2):46-9. doi: 10.1007/BF00269203.

Abstract

The influence of various macronutrients on growth and RA formation in cell suspension cultures of Anchusa officinalis has been investigated. Factors tested included sucrose concentration, alternate carbon sources, nitrate, phosphate and calcium concentration. The optimum concentration of sucrose was 3%. Fructose, glucose or their 1:1 mixture were also suitable carbon sources. The optimum concentrations of nitrate (15 mM), phosphate (3 mM) and calcium (0.25 mM) were, respectively, 3/5, 3x, and 1/4 those in normal B5 medium, when tested separately. These concentrations improved not only the yield of RA but also cell growth to a similar degree (10%-50%). Studies on the combined effects of these optimum macronutrient concentrations in B5 medium showed that RA production is inhibited by 2,4-D-containing revised medium but stimulated by NAA-containing revised medium.

摘要

研究了各种大量营养素对 Anchusa officinalis 细胞悬浮培养物生长和 RA 形成的影响。测试的因素包括蔗糖浓度、替代碳源、硝酸盐、磷酸盐和钙浓度。蔗糖的最佳浓度为 3%。果糖、葡萄糖或它们的 1:1 混合物也是合适的碳源。当分别测试时，硝酸盐（15 mM）、磷酸盐（3 mM）和钙（0.25 mM）的最佳浓度分别为正常 B5 培养基中的 3/5、3x 和 1/4，这些浓度不仅提高了 RA 的产量，而且还使细胞生长提高了相似的程度（10%-50%）。对 B5 培养基中这些最佳大量营养素浓度的组合效应的研究表明，含 2,4-D 的改良培养基抑制 RA 产生，但含 NAA 的改良培养基刺激 RA 产生。