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甘薯悬浮细胞培养中出现花青质体。

Occurrence of anthocyanoplasts in cell suspension cultures of sweet potato.

机构信息

Department of Biology, Faculty of Science, Shinshu University, 390, Matsumoto, Japan.

出版信息

Plant Cell Rep. 1985 Oct;4(5):252-5. doi: 10.1007/BF00269370.

DOI:10.1007/BF00269370
PMID:24253981
Abstract

Intensely pigmented and spherical vesicles (anthocyanoplasts) were found in anthocyanin-containing cells of sweet potato (Ipomoea batatas) suspension cultures. Anthocyanin synthesis began to first occur 24-48 h after exposure to light, and then numerous small red vesicles were detected under a microscope. The frequency of anthocyanoplast-containing cells rapidly increased to finally about 80% of the total cultured cells after 5 days of irradiation. Fully developed anthocyanoplasts reached 10-15 μm in diameter. On the other hand, neither anthocyanin synthesis nor development of anthocyanoplasts was induced in the dark-cultured cells. 2,4-D also inhibited anthocyanin synthesis and development of these vesicles. The results suggest that anthocyanoplasts might be a site of anthocyanin synthesis and/or accumulation.

摘要

在甘薯(Ipomoea batatas)悬浮培养物的含花青素细胞中发现了色素沉着和球形小泡(花青质体)。暴露于光后 24-48 小时开始合成花青素,然后在显微镜下检测到许多小红小泡。在照射 5 天后,含花青质体的细胞的频率迅速增加,最终达到总培养细胞的约 80%。完全发育的花青质体直径达到 10-15μm。另一方面,在黑暗培养的细胞中既没有诱导花青素的合成,也没有诱导花青质体的发育。2,4-D 还抑制了这些小泡的花青素合成和发育。结果表明,花青质体可能是花青素合成和/或积累的部位。

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本文引用的文献

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