USDA-ARS Western Regional Research Center, 800 Buchanan Street, 94710, Albany, California.
J Chem Ecol. 1992 Mar;18(3):333-52. doi: 10.1007/BF00994235.
The alfalfa seed chalcid (ASC),Bruchophagus roddi, is a monophagous pest of alfalfa that parasitizes developing seeds. To further understand the olfactory basis of host-plant recognition by ASCs, we recorded electroantennograms (EAGs) from females and males to 39 volatiles from both alfalfa and red clover. The chemoreceptive sensitivity of ASCs was selective for certain general classes of compounds, defined by their carbon-chain length (C6 and C8), structure (aliphatics and phenolics), isomerism, and/or functional group (acetates, ketones, and alcohols). The compounds that elicited the largest EAGs were ranked as follows: (Z)-3-hexenyl acetate > hexyl acetate ≥ acetophenone ≥ octan-3-one ≥ methyl salicylate > octan-3-ol > oct-1-en-3-one > oct-1-en-3-ol ≥ (E)-β-ocimene ≥ (Z)- and (E)-3-hexen-1-ols. Over half the test compounds elicited significantly different responses between the sexes, and female antennal responses exceeded those of males for twice the number of these volatiles. Relationships of the tested volatiles to host-plant composition, EAG responses, and ASC behaviors showed no consistent correlations. However, nearly all of the host-plant volatiles known to stimulate behavioral activity also elicited moderate to potent EAG responses.
紫花苜蓿种子寡鬃实蝇（ASC）是一种专食性害虫，以发育中的种子为食。为了进一步了解 ASC 对宿主植物的嗅觉识别基础，我们记录了雌性和雄性 ASC 对紫花苜蓿和红三叶草中 39 种挥发物的触角电位（EAG）。ASC 的化学感受灵敏度对某些特定的化合物类别具有选择性，这些化合物类别由其碳链长度（C6 和 C8）、结构（脂肪族和酚类）、异构体和/或官能团（醋酸盐、酮和醇）定义。引起最大 EAG 的化合物按以下顺序排列：（Z）-3-己烯基醋酸盐>己基醋酸盐≥苯乙酮≥辛烷-3-酮≥水杨酸甲酯>辛烷-3-醇>辛-1-烯-3-酮>辛-1-烯-3-醇≥（E）-β-罗勒烯≥（Z）-和（E）-3-己烯-1-醇。超过一半的测试化合物在两性之间引起明显不同的反应，并且这些挥发物中，雌性触角的反应超过了雄性触角的反应两倍。测试的挥发物与宿主植物组成、EAG 反应和 ASC 行为之间没有一致的关系。然而，几乎所有已知刺激行为活动的宿主植物挥发物也引起了中等至强烈的 EAG 反应。
