Qiu M, Liu J, Han C, Wu B, Yang Z, Su F, Quan F, Zhang Y
College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, China; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.
Reprod Domest Anim. 2014 Feb;49(1):170-6. doi: 10.1111/rda.12256. Epub 2013 Nov 21.
Early follicular development is closely related to oocyte-granulosa cells-ovarian stromal cells/theca cells. The aim of the present study was to investigate the effects of ovarian cortical, medullary stromal and theca cells on oestradiol and progesterone biosynthesis, proliferation and apoptosis of goat ovary granulosa cells in vitro. Using Transwell coculture system, we evaluated steroidogenesis, cell proliferation and apoptosis, and some molecular expressions regarding steroidogenic enzyme, luteinizing hormone receptor and apoptosis-related genes in granulosa cells. The results indicated that ovarian stromal/theca cells were able to stimulate oestradiol and progesterone production, promote cell proliferation and inhibit apoptosis of granulosa cells. Among all the three kinds of cells, theca cells affected strongly on granulosa cell function, and ovarian medullary stromal cells had the weakest effect on granulosa cells. These findings would provide an important knowledge of cell interaction among follicular cells during follicular development.
早期卵泡发育与卵母细胞-颗粒细胞-卵巢基质细胞/卵泡膜细胞密切相关。本研究的目的是探讨卵巢皮质、髓质基质和卵泡膜细胞对体外培养的山羊卵巢颗粒细胞雌二醇和孕酮生物合成、增殖及凋亡的影响。利用Transwell共培养系统，我们评估了颗粒细胞中的类固醇生成、细胞增殖和凋亡，以及与类固醇生成酶、促黄体生成素受体和凋亡相关基因有关的一些分子表达。结果表明，卵巢基质/卵泡膜细胞能够刺激颗粒细胞雌二醇和孕酮的产生，促进细胞增殖并抑制其凋亡。在这三种细胞中，卵泡膜细胞对颗粒细胞功能的影响最强，而卵巢髓质基质细胞对颗粒细胞的影响最弱。这些发现将为卵泡发育过程中卵泡细胞间的细胞相互作用提供重要认识。
