Hyogo Prefectural Institute of Public Health and Consumer Sciences, Kobe, Japan.
J Sep Sci. 2014 Feb;37(3):304-7. doi: 10.1002/jssc.201301132. Epub 2013 Dec 12.
This study describes a method for the simultaneous determination of 12 synthetic cannabinoids by MEKC-MS/MS using a volatile surfactant (ammonium perfluorooctanoate) as a constituent of the micellar pseudostationary phase. Although most synthetic cannabinoids comigrated by a CZE method, sufficient separation could be achieved by the proposed method. The best separation was made possible by 50 mM ammonium perfluorooctanoate in 20% v/v acetonitrile/water (apparent pH* 9.0) as the BGE, followed by MS detection using a sheath liquid composed of 5 mM ammonium formate in 50% v/v methanol/water mixed hydro-organic solvent. The standard calibration curve for all analytes showed good linearity (r > 0.99). Satisfactory recoveries, ranging from 89.5 to 101.7%, were obtained. The LODs were 6.5-76.5 μg/g for the target analytes. This method appears to be a useful tool for the identification of synthetic cannabinoids in illegal herbal incense blends.
本研究描述了一种使用挥发性表面活性剂（全氟辛基铵）作为胶束准固定相组成部分的 MEKC-MS/MS 同时测定 12 种合成大麻素的方法。虽然大多数合成大麻素通过 CZE 方法共迁移，但通过所提出的方法可以实现充分的分离。最佳分离是通过 50 mM 全氟辛基铵在 20% v/v 乙腈/水（表观 pH*9.0）作为 BGE 实现的，随后使用由 5 mM 甲酸铵在 50% v/v 甲醇/水混合有机溶剂组成的鞘液进行 MS 检测。所有分析物的标准校准曲线均显示出良好的线性（r > 0.99）。获得了令人满意的回收率，范围为 89.5 至 101.7%。目标分析物的 LOD 为 6.5-76.5μg/g。该方法似乎是鉴定非法草药焚香混合物中合成大麻素的有用工具。
