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中华倒刺鲃中肉碱棕榈酰转移酶I(CPT I)的动力学随其发育而变化。

Kinetics of carnitine palmitoyltransferase I (CPT I) in Chinese sucker (Myxocyprinus asiaticus) change with its development.

作者信息

Liu Cai-Xia, Luo Zhi, Hu Wei, Tan Xiao-Ying, Zheng Jia-Lang, Chen Qi-Liang, Zhu Qing-Ling

机构信息

Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C, Fishery College, Huazhong Agricultural University, Wuhan, 430070, China.

出版信息

Lipids. 2014 Feb;49(2):173-81. doi: 10.1007/s11745-013-3864-5. Epub 2013 Nov 22.

Abstract

The present study was conducted to evaluate the ontogeny and kinetic of CPT I in several tissues of the Chinese sucker Myxocyprinus asiaticus. To this end, liver, muscle and intestine tissues were examined at five various developmental stages of Chinese sucker: newly-hatched larvae, 68-day-old, 4-month-old, 1- and 2-year-old Chinese sucker, respectively. The total carnitine (TC) content in the liver increased from birth to 1-year-old Chinese sucker and then declined in the 2-year-old Chinese sucker. From the 68-day-old to the 1-year-old Chinese sucker, both free and total concentrations in muscle increased. Both acyl carnitine (AC) and TC concentrations in intestine were very variable at different stages. The ratio of AC to free carnitine (FC) in liver progressively increased from hatching to 4 months, and declined at the age of 1 year, and then increased by 2 years. In muscle, the highest and lowest ratios of AC/FC were observed at 68-days-old and 4 month-old Chinese sucker. The highest proportion of AC/FC in intestine was also observed at 68-day-old Chinese sucker. All the Chinese sucker larvae at hatching had a high value of the Michaelis constant (K(m)) for carnitine. Maximal velocity (V(max)) in intestine increased with age. V(max) in liver and muscle increased with age except a decrease at 4 months in liver and at 2 years in muscle. The FC concentration in the examined tissues at all developmental stages were less than the respective K(m), indicating that Chinese suckers require supplemental carnitine in their food to ensure that CPT I activity is not constrained by carnitine availability.

摘要

本研究旨在评估中华倒刺鲃(Myxocyprinus asiaticus)几种组织中肉碱棕榈酰转移酶I(CPT I)的个体发生和动力学。为此,在中华倒刺鲃的五个不同发育阶段对肝脏、肌肉和肠道组织进行了检查:刚孵化的幼体、68日龄、4月龄、1岁和2岁的中华倒刺鲃。肝脏中的总肉碱(TC)含量从出生到1岁的中华倒刺鲃增加,然后在2岁的中华倒刺鲃中下降。从68日龄到1岁的中华倒刺鲃,肌肉中的游离和总浓度均增加。肠道中的酰基肉碱(AC)和TC浓度在不同阶段变化很大。肝脏中AC与游离肉碱(FC)的比值从孵化到4个月逐渐增加,在1岁时下降,然后在2岁时增加。在肌肉中,AC/FC的最高和最低比值分别在68日龄和4月龄的中华倒刺鲃中观察到。肠道中AC/FC的最高比例也在68日龄的中华倒刺鲃中观察到。所有孵化时的中华倒刺鲃幼体对肉碱的米氏常数(K(m))值都很高。肠道中的最大速度(V(max))随年龄增加。肝脏和肌肉中的V(max)随年龄增加,但肝脏在4个月时和肌肉在2岁时有所下降。所有发育阶段检查组织中的FC浓度均低于各自的K(m),表明中华倒刺鲃需要在食物中补充肉碱,以确保CPT I活性不受肉碱可用性的限制。

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