Clinical Bacteriology Laboratory, Indian Veterinary Research Institute (IVRI), Mukteswar-263138, Nainital (District), Uttarakhand (UK), India.
Clinical Bacteriology Laboratory, Indian Veterinary Research Institute (IVRI), Mukteswar-263138, Nainital (District), Uttarakhand (UK), India.
Vaccine. 2014 Jan 3;32(2):290-6. doi: 10.1016/j.vaccine.2013.10.075. Epub 2013 Nov 23.
Bacterial lipoproteins are emerging targets for inducing protective immunity against many infectious diseases. VacJ is a highly conserved and widely distributed outer membrane lipoprotein of Pasteurella multocida strains, which are known to affect a wide range of domestic as well as wild animals and birds. In the present study, the gene encoding for mature VacJ outer membrane lipoprotein of P. multocida serogroup B:2 strain P52 was cloned and over-expressed in Escherichia coli as a fusion protein. The purified recombinant VacJ protein (∼44kDa) was used for immunizing mice (6/group) along with adjuvants (FCA and alum) in two experiments. Immunization of mice with rVacJ (30μg and 75μg/mice) elicited humoral immune response with significant (P<0.01) rise in antigen-specific titers of IgG and its subtypes (IgG1 and IgG2a). No protection was noticed in mice immunized with rVacJ (30μg) along with FCA followed by challenge with 100 LD50 of the homologous strain. On the contrary, higher rVacJ dose (75μg) along with FCA and alum provided 66.7% and 50% protection respectively, at reduced challenge dose (8 LD50). The study indicated that a lipidated recombinant VacJ lipoprotein with suitable adjuvants could potentially act as candidate antigen for vaccine development against pasteurellosis in livestock.
细菌脂蛋白是诱导针对许多传染病的保护性免疫的新兴靶标。VacJ 是巴氏杆菌属菌株中高度保守且广泛分布的外膜脂蛋白，已知其会影响多种家养和野生动物和鸟类。在本研究中，克隆并在大肠杆菌中作为融合蛋白过表达了巴氏杆菌 B：2 血清型 P52 菌株的成熟 VacJ 外膜脂蛋白编码基因。纯化的重组 VacJ 蛋白（约 44kDa）与佐剂（FCA 和明矾）一起用于两种实验中的免疫小鼠（每组 6 只）。用 rVacJ（30μg 和 75μg/只）免疫小鼠可引起体液免疫应答，抗原特异性 IgG 及其亚型（IgG1 和 IgG2a）滴度显著升高（P<0.01）。用 rVacJ（30μg）与 FCA 免疫小鼠，然后用同源株 100LD50 进行攻毒，未观察到保护作用。相反，较高剂量的 rVacJ（75μg）与 FCA 和明矾一起提供了 66.7%和 50%的保护率，攻毒剂量降低（8 LD50）。该研究表明，具有合适佐剂的脂化重组 VacJ 脂蛋白可能成为针对家畜巴氏杆菌病疫苗开发的候选抗原。
