儿童皮肤中II类细胞因子基因的表达。
Expression of class II cytokine genes in children's skin.
作者信息
Reemann Paula, Reimann Ene, Suutre Siim, Paavo Maarjaliis, Loite Ulvi, Porosaar Orm, Abram Kristi, Silm Helgi, Vasar Eero, Kõks Sulev, Kingo Külli
机构信息
Department of Physiology, University of Tartu, Ravila 19, 50411 Tartu, Estonia.
出版信息
Acta Derm Venereol. 2014 Jul;94(4):386-92. doi: 10.2340/00015555-1717.
Immune regulation of the skin plays an important role in susceptibility and development of illnesses. The aim of our study was to localise the interleukin (IL)-10 family of cytokines, in children's skin and to determine possible age-related differences in the expression level. The mRNA expression level of IL10, IL19, IL20, IL22, IL24, IL26, IL28B, IL29 and their receptors IL10RA, IL10RB, IL20RA, IL20RB, IL22RA1, IL22RA2, IL28RA was compared in skin biopsies of children and adults and in childrens' skin cells by quantitative real-time PCR (qRT-PCR). Immunohistochemistry was performed to confirm the qRT-PCR findings. We found age-related differences in the expression of IL10RB, IL20, IL20RA, IL22RA1, IL22RA2, IL26 and IL28RA genes. Cell type-dependent expression of IL10 family cytokines was apparent in the skin. In addition to previously known differences in systemic immunological response of adults and children, the present results reveal differences in immune profile of adult and juvenile skin.
皮肤的免疫调节在疾病的易感性和发展过程中起着重要作用。我们研究的目的是在儿童皮肤中定位白细胞介素（IL）-10细胞因子家族，并确定表达水平中可能存在的年龄相关差异。通过定量实时聚合酶链反应（qRT-PCR）比较儿童和成人皮肤活检组织以及儿童皮肤细胞中IL10、IL19、IL20、IL22、IL24、IL26、IL28B、IL29及其受体IL10RA、IL10RB、IL20RA、IL20RB、IL22RA1、IL22RA2、IL28RA的mRNA表达水平。进行免疫组织化学以证实qRT-PCR的结果。我们发现IL10RB、IL20、IL20RA、IL22RA1、IL22RA2、IL26和IL28RA基因的表达存在年龄相关差异。IL10家族细胞因子的细胞类型依赖性表达在皮肤中很明显。除了成人和儿童在全身免疫反应方面先前已知的差异外，目前的结果还揭示了成人和青少年皮肤免疫特征的差异。
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