Department of Otolaryngology/Head and Neck Surgery, University Hospital Giessen and Marburg, Campus Marburg, Baldingerstrasse, D-35033 Marburg, Germany.
In Vivo. 2013 Nov-Dec;27(6):701-5.
The present feasibility study evaluated the chorioallantoic membrane (CAM) assay established in cancer and angiogenesis research as a tool for the study of vascular anomalies (VAs) in the head and neck area, since the lack of appropriate model systems poses a major obstacle in VA research.
VA tissues from three patients, two with an arteriovenous and one with a lymphatic malformation, were analyzed and evaluated in the CAM assay.
The arteriovenous malformations induced a potent angiogenic reaction, resulting in new vessel growth and reperfusion by chicken embryo blood, which was comparable in extent with the positive vascular endothelial growth factor control. An angiogenic reaction, although less pronounced, was also observed in the single-tested lymphatic malformation.
Our observations indicate the CAM assay to be a suitable model system for the study of VAs, as well as to show how treatment with pro- and antiangiogenic drugs affects VA growth patterns. The CAM assay has the potential to become a valuable tool for VA studies.
本可行性研究评估了在癌症和血管生成研究中建立的鸡胚尿囊膜（CAM）检测法，作为研究头颈部血管异常（VA）的一种工具，因为缺乏合适的模型系统是 VA 研究的主要障碍。
对来自三名患者的 VA 组织进行了分析和评估，两名患者为动静脉畸形，一名患者为淋巴管畸形。
动静脉畸形诱导了强烈的血管生成反应，导致新血管生长和鸡胚血液再灌注，其程度与阳性血管内皮生长因子对照相当。在单次检测的淋巴管畸形中也观察到了较弱的血管生成反应。
我们的观察表明，CAM 检测法是研究 VA 的合适模型系统，同时也表明了使用促血管生成和抗血管生成药物治疗如何影响 VA 的生长模式。CAM 检测法有可能成为 VA 研究的一种有价值的工具。
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