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鱼汤对乳酸菌生物胺形成的影响。

The influences of fish infusion broth on the biogenic amines formation by lactic acid bacteria.

机构信息

Department of Seafood Processing Technology, Faculty of Fisheries, Cukurova University, Adana, Turkey.

出版信息

Braz J Microbiol. 2013 Oct 30;44(2):407-15. doi: 10.1590/S1517-83822013000200010. eCollection 2013.

Abstract

The influences of fish infusion decarboxylase broth (IDB) on biogenic amines (BA) formation by lactic acid bacteria (LAB) were investigated. BA productions by single LAB strains were tested in five different fish (anchovy, mackerel, white shark, sardine and gilthead seabream) IDB. The result of the study showed that significant differences in ammonia (AMN) and BA production were observed among the LAB strains in fish IDB (p < 0.05). The highest AMN and TMA production by LAB strains were observed for white shark IDB. The all tested bacteria had decarboxylation activity in fish IDB. The uppermost accumulated amines by LAB strains were tyramine (TYM), dopamine, serotonin and spermidine. The maximum histamine production was observed in sardine (101.69 mg/L) and mackerel (100.84 mg/L) IDB by Leuconostoc mesenteroides subsp. cremoris and Pediococcus acidophilus, respectively. Lactobacillus delbrueckii subsp. lactis and Pediococcus acidophilus had a high TYM producing capability (2943 mg/L and 1157 mg/L) in sardine IDB.

摘要

研究了鱼浸液脱羧酶肉汤(IDB)对乳酸菌(LAB)生物胺(BA)形成的影响。在五种不同的鱼(凤尾鱼、鲭鱼、白鲨、沙丁鱼和金头鲷)IDB 中测试了单一 LAB 菌株的 BA 产量。研究结果表明,在鱼 IDB 中,LAB 菌株的氨(AMN)和 BA 产量存在显著差异(p < 0.05)。白鲨 IDB 中 LAB 菌株的 AMN 和 TMA 产量最高。所有测试的细菌在鱼 IDB 中都具有脱羧活性。LAB 菌株积累的最高胺是酪胺(TYM)、多巴胺、血清素和亚精胺。在凤尾鱼(101.69 mg/L)和鲭鱼(100.84 mg/L)IDB 中,分别由肠膜明串珠菌亚种。乳脂乳球菌和嗜酸乳杆菌产生的组胺产量最高。在沙丁鱼 IDB 中,德氏乳杆菌亚种。乳脂乳球菌和嗜酸乳杆菌具有较高的 TYM 产生能力(2943 mg/L 和 1157 mg/L)。

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