Department of Pathology (C.P., J.V.), San Agustín Hospital, University Institute of Oncology of the Principality of Asturias (IUOPA-HUCA) Gynaecology Department (M.C.), Jarrio Hospital, Coaña, SESPA, Asturias Unit of Infections and Cancer, Cancer Epidemiology Research Programme (L.A., S.T., J.K., S.S.), IDIBELL, Catalan Institute of Oncology (ICO), L'Hospitalet de Llobregat CIBER Epidemiology and Public Health (CIBERESP) (S.S.), Institut Catalan Institute of Oncology (ICO), L'Hospitalet de Llobregat, Barcelona, Spain.
Int J Gynecol Pathol. 2014 Jan;33(1):74-82. doi: 10.1097/PGP.0b013e3182774546.
The aims of this study were to assess the overexpression of p16 in formalin-fixed paraffin-embedded cervical carcinoma tissue blocks and to determine its concordance with the human papillomavirus (HPV) status using the SPF10-LiPA25 polymerase chain reaction System and its correlation with the histologic type of invasive cervical cancer (ICC) and individual HPV genotypes. A total of 205 retrospectively collected ICC cases were analyzed by p16 immunohistochemistry. HPV detection was performed by polymerase chain reaction using SPF10 broad-spectrum primers, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse hybridization line probe assay (LiPA25). Of 205, 188 analyzed (91.7%) ICC cases showed p16 overexpression, whereas 181 (83.3%) cases were HPV positive using HPV LiPA testing. One hundred and seventy four (84.9%) cases were both p16 and HPV LiPA positive, indicating a positive concordance of 89.9% between both techniques (κ index agreement of 0.43; P<0.001), and no statistically significant difference (McNemar test, P>0.05). Squamous cell carcinomas were strongly positive compared with the adenocarcinomas (93.6% vs. 75% of the cases, respectively). When performed on formalin-fixed paraffin-embedded cervical tissue specimens, the higher positivity rate of p16 immunohistochemistry as compared with HPV DNA testing may allow identifying HPV-related ICC cases in which HPV testing was negative.
本研究旨在评估 p16 在福尔马林固定石蜡包埋的宫颈癌组织块中的过表达情况，并使用 SPF10-LiPA25 聚合酶链反应系统确定其与人类乳头瘤病毒（HPV）状态的一致性，以及与浸润性宫颈癌（ICC）的组织学类型和个体 HPV 基因型的相关性。对 205 例回顾性收集的 ICC 病例进行 p16 免疫组化分析。采用 SPF10 广谱引物进行聚合酶链反应检测 HPV，然后进行脱氧核糖核酸酶免疫测定和反向杂交线探针分析（LiPA25）进行基因分型。在 205 例 ICC 病例中，188 例（91.7%）病例显示 p16 过表达，而 181 例（83.3%）病例使用 HPV LiPA 检测呈 HPV 阳性。174 例（84.9%）病例均为 p16 和 HPV LiPA 阳性，两种技术的阳性一致性为 89.9%（κ 指数一致性为 0.43；P<0.001），且无统计学差异（McNemar 检验，P>0.05）。与腺癌相比，鳞状细胞癌的阳性率更高（分别为 93.6%和 75%）。与 HPV DNA 检测相比，在福尔马林固定石蜡包埋的宫颈组织标本上进行 p16 免疫组化的阳性率更高，可能有助于识别 HPV 检测阴性的 HPV 相关 ICC 病例。
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