中国荷斯坦奶牛中催乳素-RsaI（PRL-RsaI）基因座的新见解及其对产奶性能性状的影响。

New insights into the prolactin-RsaI (PRL-RsaI) locus in Chinese Holstein cows and its effect on milk performance traits.

作者信息

Dong C H, Song X M, Zhang L, Jiang J F, Zhou J P, Jiang Y Q

机构信息

Agricultural Science and Engineering Department, Shandong Agriculture and Engineering University, Jinan, Shandong, China.

出版信息

Genet Mol Res. 2013 Nov 22;12(4):5766-73. doi: 10.4238/2013.November.22.3.

DOI:10.4238/2013.November.22.3

PMID:24301945

Abstract

Prolactin (PRL) plays central roles in mammals' reproduction, gland development, milk secretion, and the expression of milk protein genes. In dairy cattle, the PRL gene is a potential quantitative trait locus and genetic marker related to milk performance traits. Here, a total of 586 randomly selected Chinese Holstein cows were genotyped for locus PRL-RsaI. One haplotype block containing eight SNPs was identified in the region from intron 3 to intron 4 of the PRL gene in Chinese Holstein cows. One tag SNP (7545 G → A) was selected to represent the haplotype block defined by the genotypic data. The cows with genotype AA of this tag SNP had a higher milk yield at 305 days (8457 ± 938 kg) than the cows with GA (7537 ± 1278 kg; P < 0.01) or GG (7757 ± 1174 kg; P < 0.05). This suggests that the haplotype block examined in this study contains important markers for milk production traits in Chinese Holstein cows.

摘要

催乳素（PRL）在哺乳动物的繁殖、腺体发育、乳汁分泌以及乳蛋白基因的表达中发挥着核心作用。在奶牛中，PRL基因是一个与产奶性能性状相关的潜在数量性状位点和遗传标记。在此，总共对586头随机选取的中国荷斯坦奶牛进行了PRL-RsaI位点的基因分型。在中国荷斯坦奶牛的PRL基因从内含子3到内含子4的区域中，鉴定出一个包含8个单核苷酸多态性（SNP）的单倍型块。选择一个标签单核苷酸多态性（7545 G→A）来代表由基因型数据定义的单倍型块。该标签单核苷酸多态性基因型为AA的奶牛在305天时的产奶量（8457±938千克）高于基因型为GA的奶牛（7537±1278千克；P<0.01）或GG的奶牛（7757±1174千克；P<0.05）。这表明本研究中检测的单倍型块包含中国荷斯坦奶牛产奶性状的重要标记。